ANTIFUNGAL ACTIVITY OF CLOVE AND CINNAMON MEDIATED SELENIUM NANOPARTICLES: AN In vitro STUDY
PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, Volume 21, Issue 23-24,
Page 18-23
Abstract
Background: Cinnamon and clove are both natural preservative and flavoring substances that are not harmful when consumed in food products. Selenium nanoparticles (SeNPs) are the nanoscale metal structures. SeNPs have exhibited great antimicrobial activities against fungi and bacteria.
Aim: To determine the antifungal activity of clove and cinnamon mediated selenium nanoparticles against Candida albicans.
Materials and Methods: The clove and cinnamon powder was procured. An aqueous extract was prepared and mixed with sodium selenide precursor for selenium nanoparticle formation and centrifuged at 8000 rpm for 10 minutes. The extract was then placed in the well cultured agar plates against Candida albicans and incubated for 24 hours at 37ºC. The zones of inhibition were calculated by disk diffusion method.
Results: Cinnamon and clove mediated selenium nanoparticles have good antifungal activity with the zone of inhibition of 35 mm at the highest concentration (150 µl). Whereas, the control Amphotericin B showed a zone of inhibition of 34 mm.
Conclusion: Cinnamon and clove mediated selenium nanoparticles were studied and their wider zones of inhibition were suggestive of good antifungal efficiency.
Keywords:
- Clove
- cinnamon
- Candida albicans
- selenium nanoparticle
How to Cite
KUMAR, A., ARVINA, R., & RAJESHKUMAR, S. (2020). ANTIFUNGAL ACTIVITY OF CLOVE AND CINNAMON MEDIATED SELENIUM NANOPARTICLES: AN In vitro STUDY. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(23-24), 18–23. Retrieved from https://ikprress.org/index.php/PCBMB/article/view/5289
References
Iravani S. Green synthesis of metal nanoparticles using plants. Green Chem 2011;13:2638–2650.
Cornistein W, Mora A, Orellana N, et al. Candida: Epidemiology and risk factors for non-albicans species. EnfermInfecc Microbiol Clin. 2013;31:380–384.
Lockhart SR, Iqbal N, Cleveland AA. Species identification and antifungal susceptibility testing of Candida bloodstream isolates from population-based surveillance studies in two US cities from 2008 to. Journal of Clinical; 2012.
Available:https://jcm.asm.org/content/50/11/3435.short
Kashyap PL, Kumar S, Srivastava AK, et al. Myconanotechnology in agriculture: A perspective. World J Microbiol Biotechnol. 2013;29:191–207.
Panáček A, Kolář M, Večeřová R, et al. Antifungal activity of silver nanoparticles against Candida spp. Biomaterials. 2009; 30:6333–6340.
Shenashen MA, El-Safty SA, Elshehy EA. Synthesis, morphological control, and properties of silver nanoparticles in potential applications. Part Part Syst Charact. 2014;31:293–316.
Sintubin L, Verstraete W, Boon N. Biologically produced nanosilver: Current state and future perspectives. Biotechnol Bioeng. 2012;109:2422–2436.
Quester K, Avalos-Borja M, Castro-Longoria E. Biosynthesis and microscopic study of metallic nanoparticles. Micron. 2013;54-55:1–27.
Mihaliak CA, Gershenzon J, Croteau R. Lack of rapid monoterpene turnover in rooted plants: Implications for theories of plant chemical defense. Oecologia. 1991; 87:373–376.
Matan N, Rimkeeree H, Mawson AJ, et al. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. Int J Food Microbiol. 2006; 107:180–185.
Ezhilarasan D, Apoorva VS, Ashok Vardhan N. Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells. J Oral Pathol Med. 2019; 48:115–121.
Gajendran PL, Parthasarathy H, Tadepalli A. Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitis. Indian J Dent Res. 2018;29:588–593.
Kaarthikeyan G, Jayakumar ND, Sivakumar D. Comparative evaluation of bone formation between PRF and blood clot alone as the sole sinus-filling material in maxillary sinus augmentation with the implant as a tent pole: A randomized split-mouth study. J Long Term Eff Med Implants. 2019;29:105–111.
Arjunkumar R. Nanomaterials for the Management of periodontal diseases. In: Chaughule RS (ed) Dental applications of nanotechnology. Cham: Springer International Publishing. 2018;203–215.
Ravi S, Malaiappan S, Varghese S, et al. Additive effect of plasma rich in growth factors with guided tissue regeneration in treatment of intrabony defects in patients with chronic periodontitis: A split-mouth randomized controlled clinical trial. J Periodontol. 2017;88:839–845.
Kavarthapu A, Malaiappan S. Comparative evaluation of demineralized bone matrix and type II collagen membrane versus eggshell powder as a graft material and membrane in rat model. Indian J Dent Res. 2019;30:877–880.
Murthykumar K, Arjunkumar R, Jayaseelan VP. Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis. J Investig Clin Dent. 2019; 10:12440.
Ramesh A, Vellayappan R, Ravi S, et al. Esthetic lip repositioning: A cosmetic approach for correction of gummy smile - A case series. J Indian Soc Periodontol. 2019;23:290–294.
Ramesh A, Varghese S, Jayakumar ND, et al. Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study. J Periodontol. 2018;89:1241–1248.
Kavarthapu A, Thamaraiselvan M. Assessing the variation in course and position of inferior alveolar nerve among south Indian population: A cone beam computed tomographic study. Indian J Dent Res. 2018;29:405–409.
Ramesh A, Ravi S, Kaarthikeyan G. Comprehensive rehabilitation using dental implants in generalized aggressive periodontitis. J Indian Soc Periodontol. 2017;21:160–163.
Jain M, Nazar N. Comparative Evaluation of the Efficacy of intraligamentary and supraperiosteal injections in the extraction of maxillary teeth: A randomized controlled clinical trial. J Contemp Dent Pract. 2018; 19:1117–1121.
Vijayashree Priyadharsini J. In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens. J Periodontol. 2019;90:1441–1448.
Rajeshkumar S. Green synthesis of different sized antimicrobial silver nanoparticles using different parts of plants-A Review. International Journal of ChemTech Research. 2016;9:197–208.
Happy Agarwal, Soumya Menon, Venkat Kumar S, et al. Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route. Chem Biol Interact. 2018;286:60–70.
Rajeshkumar S, Bharath LV. Mechanism of plant-mediated synthesis of silver nanoparticles--a review on biomolecules involved, characterisation and antibacterial activity. Chem Biol Interact. 2017;273: 219–227.
Santhoshkumar J, Rajeshkumar S, Kumar SV. Phyto-assisted synthesis, characterization and applications of gold nanoparticles- A review. Biochemistry and Biophysics Reports 2017;11:46–57.
Menon S, SR, SVK. A review on biogenic synthesis of gold nanoparticles, characterization, and its applications. Resource-Efficient Technologies. 2017;3: 516–527.
Rajeshkumar S, Naik P. Synthesis and biomedical applications of cerium oxide nanoparticles- A review. Biotechnology Reports. 2018;17:1–5.
Agarwal H, Kumar SV, Rajeshkumar S. A review on green synthesis of zinc oxide nanoparticles: An eco-friendly approach. ResourEffic Technol. 2017;3(4):406--413.
Menon S, Ks SD, RS, et al. Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism. Colloids Surf B Biointerfaces. 2018;170: 280–292.
Kim JS, Kuk E, Yu KN, et al. Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007;3:95–101.
Menezes EA, dos Santos Oliveira M da C, Cunha FA, et al. Identificação preliminar de algumasespécies do gênero Candida spp. emmeiocromógeno: Resultados de doisanos de um estudo multicêntrico realizado no Ceará. Revista de Patologia Tropical/ Journal of Tropical Pathology. 2011;40: 297–303.
Azzouz MA, Bullerman LB. Comparative antimycotic effects of selected herbs, spices, plant components and commercial antifungal agents. J Food Prot. 1982;45: 1298–1301.
Soliman KM, Badeaa RI. Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food Chem Toxicol 2002;40:1669–1675.
Velluti A, Sanchis V, Ramos AJ, et al. Inhibitory effect of cinnamon, clove, lemongrass, oregano and palmarose essential oils on growth and fumonisin B1 production by Fusarium proliferatum in maize grain. Int J Food Microbiol. 2003; 89:145–154.
López-Malo A, Barreto-Valdivieso J, Palou E, et al. Aspergillus flavus growth response to cinnamon extract and sodium benzoate mixtures. Food Control. 2007;18:1358–1362.
Feng W, Zheng X. Essential oils to control Alternaria alternata in vitro and In vivo. Food Control. 2007; 18:1126–1130.
Wang SY, Chen PF, Chang ST. Antifungal activities of essential oils and their constituents from indigenous cinnamon (Cinnamomum osmophloeum) leaves against wood decay fungi. Bioresour Technol 2005;96:813–818.
Farag RS, Daw ZY, Abo-Raya SH. Influence of some spice essential oils on Aspergillus parasiticus growth and production of aflatoxins in a Synthetic Medium. J Food Sci. 1989;54: 74–76.
Bang KH, Lee DW, Park HM, et al. Inhibition of Fungal cell wall synthesizing enzymes by trans-Cinnamaldehyde. Biosci Biotechnol Biochem. 2000;64:1061–1063.
Cornistein W, Mora A, Orellana N, et al. Candida: Epidemiology and risk factors for non-albicans species. EnfermInfecc Microbiol Clin. 2013;31:380–384.
Lockhart SR, Iqbal N, Cleveland AA. Species identification and antifungal susceptibility testing of Candida bloodstream isolates from population-based surveillance studies in two US cities from 2008 to. Journal of Clinical; 2012.
Available:https://jcm.asm.org/content/50/11/3435.short
Kashyap PL, Kumar S, Srivastava AK, et al. Myconanotechnology in agriculture: A perspective. World J Microbiol Biotechnol. 2013;29:191–207.
Panáček A, Kolář M, Večeřová R, et al. Antifungal activity of silver nanoparticles against Candida spp. Biomaterials. 2009; 30:6333–6340.
Shenashen MA, El-Safty SA, Elshehy EA. Synthesis, morphological control, and properties of silver nanoparticles in potential applications. Part Part Syst Charact. 2014;31:293–316.
Sintubin L, Verstraete W, Boon N. Biologically produced nanosilver: Current state and future perspectives. Biotechnol Bioeng. 2012;109:2422–2436.
Quester K, Avalos-Borja M, Castro-Longoria E. Biosynthesis and microscopic study of metallic nanoparticles. Micron. 2013;54-55:1–27.
Mihaliak CA, Gershenzon J, Croteau R. Lack of rapid monoterpene turnover in rooted plants: Implications for theories of plant chemical defense. Oecologia. 1991; 87:373–376.
Matan N, Rimkeeree H, Mawson AJ, et al. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. Int J Food Microbiol. 2006; 107:180–185.
Ezhilarasan D, Apoorva VS, Ashok Vardhan N. Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells. J Oral Pathol Med. 2019; 48:115–121.
Gajendran PL, Parthasarathy H, Tadepalli A. Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitis. Indian J Dent Res. 2018;29:588–593.
Kaarthikeyan G, Jayakumar ND, Sivakumar D. Comparative evaluation of bone formation between PRF and blood clot alone as the sole sinus-filling material in maxillary sinus augmentation with the implant as a tent pole: A randomized split-mouth study. J Long Term Eff Med Implants. 2019;29:105–111.
Arjunkumar R. Nanomaterials for the Management of periodontal diseases. In: Chaughule RS (ed) Dental applications of nanotechnology. Cham: Springer International Publishing. 2018;203–215.
Ravi S, Malaiappan S, Varghese S, et al. Additive effect of plasma rich in growth factors with guided tissue regeneration in treatment of intrabony defects in patients with chronic periodontitis: A split-mouth randomized controlled clinical trial. J Periodontol. 2017;88:839–845.
Kavarthapu A, Malaiappan S. Comparative evaluation of demineralized bone matrix and type II collagen membrane versus eggshell powder as a graft material and membrane in rat model. Indian J Dent Res. 2019;30:877–880.
Murthykumar K, Arjunkumar R, Jayaseelan VP. Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis. J Investig Clin Dent. 2019; 10:12440.
Ramesh A, Vellayappan R, Ravi S, et al. Esthetic lip repositioning: A cosmetic approach for correction of gummy smile - A case series. J Indian Soc Periodontol. 2019;23:290–294.
Ramesh A, Varghese S, Jayakumar ND, et al. Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study. J Periodontol. 2018;89:1241–1248.
Kavarthapu A, Thamaraiselvan M. Assessing the variation in course and position of inferior alveolar nerve among south Indian population: A cone beam computed tomographic study. Indian J Dent Res. 2018;29:405–409.
Ramesh A, Ravi S, Kaarthikeyan G. Comprehensive rehabilitation using dental implants in generalized aggressive periodontitis. J Indian Soc Periodontol. 2017;21:160–163.
Jain M, Nazar N. Comparative Evaluation of the Efficacy of intraligamentary and supraperiosteal injections in the extraction of maxillary teeth: A randomized controlled clinical trial. J Contemp Dent Pract. 2018; 19:1117–1121.
Vijayashree Priyadharsini J. In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens. J Periodontol. 2019;90:1441–1448.
Rajeshkumar S. Green synthesis of different sized antimicrobial silver nanoparticles using different parts of plants-A Review. International Journal of ChemTech Research. 2016;9:197–208.
Happy Agarwal, Soumya Menon, Venkat Kumar S, et al. Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route. Chem Biol Interact. 2018;286:60–70.
Rajeshkumar S, Bharath LV. Mechanism of plant-mediated synthesis of silver nanoparticles--a review on biomolecules involved, characterisation and antibacterial activity. Chem Biol Interact. 2017;273: 219–227.
Santhoshkumar J, Rajeshkumar S, Kumar SV. Phyto-assisted synthesis, characterization and applications of gold nanoparticles- A review. Biochemistry and Biophysics Reports 2017;11:46–57.
Menon S, SR, SVK. A review on biogenic synthesis of gold nanoparticles, characterization, and its applications. Resource-Efficient Technologies. 2017;3: 516–527.
Rajeshkumar S, Naik P. Synthesis and biomedical applications of cerium oxide nanoparticles- A review. Biotechnology Reports. 2018;17:1–5.
Agarwal H, Kumar SV, Rajeshkumar S. A review on green synthesis of zinc oxide nanoparticles: An eco-friendly approach. ResourEffic Technol. 2017;3(4):406--413.
Menon S, Ks SD, RS, et al. Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism. Colloids Surf B Biointerfaces. 2018;170: 280–292.
Kim JS, Kuk E, Yu KN, et al. Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007;3:95–101.
Menezes EA, dos Santos Oliveira M da C, Cunha FA, et al. Identificação preliminar de algumasespécies do gênero Candida spp. emmeiocromógeno: Resultados de doisanos de um estudo multicêntrico realizado no Ceará. Revista de Patologia Tropical/ Journal of Tropical Pathology. 2011;40: 297–303.
Azzouz MA, Bullerman LB. Comparative antimycotic effects of selected herbs, spices, plant components and commercial antifungal agents. J Food Prot. 1982;45: 1298–1301.
Soliman KM, Badeaa RI. Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food Chem Toxicol 2002;40:1669–1675.
Velluti A, Sanchis V, Ramos AJ, et al. Inhibitory effect of cinnamon, clove, lemongrass, oregano and palmarose essential oils on growth and fumonisin B1 production by Fusarium proliferatum in maize grain. Int J Food Microbiol. 2003; 89:145–154.
López-Malo A, Barreto-Valdivieso J, Palou E, et al. Aspergillus flavus growth response to cinnamon extract and sodium benzoate mixtures. Food Control. 2007;18:1358–1362.
Feng W, Zheng X. Essential oils to control Alternaria alternata in vitro and In vivo. Food Control. 2007; 18:1126–1130.
Wang SY, Chen PF, Chang ST. Antifungal activities of essential oils and their constituents from indigenous cinnamon (Cinnamomum osmophloeum) leaves against wood decay fungi. Bioresour Technol 2005;96:813–818.
Farag RS, Daw ZY, Abo-Raya SH. Influence of some spice essential oils on Aspergillus parasiticus growth and production of aflatoxins in a Synthetic Medium. J Food Sci. 1989;54: 74–76.
Bang KH, Lee DW, Park HM, et al. Inhibition of Fungal cell wall synthesizing enzymes by trans-Cinnamaldehyde. Biosci Biotechnol Biochem. 2000;64:1061–1063.
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