ANTI-INFLAMMATORY ACTIVITY OF TITANIUM DIOXIDE NANOPARTICLES SYNTHESISED USING GRAPE SEED EXTRACT: AN in vitro STUDY
ANKITA KOMAL LABH
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
ARVINA RAJASEKAR
Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
S. RAJESHKUMAR
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai -600 077, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Titanium dioxide nanoparticles (TiO2 NPs) synthesized from plant sources are eco-friendly, cost effective and have a wide range of applications. The anti-inflammatory property of titanium dioxide produced by various herbal sources has well been studied. Grape seeds have been used in this study to produce TiO2 NPs due to their anti-inflammatory, anti-diabetic, anti-cholesterol properties.
Aim: The aim of this study was to determine the anti-inflammatory activity of titanium dioxide nanoparticles synthesised using grape seed extract.
Materials and Methods: Preparation of grape seed extract, synthesis of titanium dioxide nanoparticles using grape seed extract in Lark refrigerated centrifuge, preparation of nanoparticles powder, characterisation using UV-Vis Spectroscopy and study of anti-inflammatory activity using Bovine serum albumin using Diclofenac sodium as control.
Results: The peak found in spectroscopy indicated the formation of titanium dioxide nanoparticles. The anti-inflammatory action of grape seed mediated TiO2 NPs increases with an increase in the concentration from 10 μl, 20 μl, 30 μl, 40 μl to 50 μl.
Conclusion: Titanium dioxide nanoparticles produced using grape seed extract have good anti-inflammatory activity and can be used in the future as pastes, gels etc. for topical application instead of using conventional anti-inflammatory agents to minimize their side effects.
Keywords: Anti-inflammatory, grape seed, herbal synthesis, nanotechnology, nanoparticles