ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF LYCOPENE EXTRACT AGAINST ORAL PATHOGENS
J. SARVESH KUMAR
Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Chennai, Tamil Nadu, India.
PRIYA LOCHANA GAJENDRAN
Department of Periodontics, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Chennai, Tamil Nadu, India.
RADHIKA ARJUNKUMAR
Department of Periodontics, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Chennai, Tamil Nadu, India.
S. RAJESHKUMAR
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Science, Chennai, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: The aim of the study was to assess the antioxidant and antimicrobial activity of lycopene extract against oral pathogens.
Materials and Methods: Lycopene extract was prepared and the antimicrobial activity was tested against Candida albicans, Streptococcus mutans (gram +), and Lactobacillus sp. 2,2-diphenylpicrylhydrazyl ( DPPH )radical scavenging assay was used to test antioxidant activity. 2 ml of lycopene extract in the concentration range of 10-50 μl was prepared, Absorbance value was measured spectrophotometrically at 517 nm. To measure the antioxidant activity the DPPH radical scavenging assay was used. 2 ml of lycopene extract in the concentration range of 10-50 μl was prepared in 50% methanol solution and was added to equal volume of 0.1 mM of DPPH solution. The reaction mixture was incubated for 30 min in dark at room temperature. Absorbance value was measured spectrophotometrically at 517 nm.
Results: From present study we can infer that for antimicrobial activity the zone of inhibition of lycopene at a concentration of 50 µl was 8 for S. mutans, Lactobacillus and Candida, at concentration of 100 µl was 9,10 and 8 for S. mutans, Lactobacillus and Candida respectively, at concentration of 150 µl was 13,14 and 9 for S. mutans, Lactobacillus and Candida respectively. The zone inhibition of lycopene was maximum for lactobacillus at concentration of 150 µl.
Conclusion: Lycopene with increase in concentration has increased antioxidant activity but was comparatively less when compared to the standard ascorbic acid. Even Though lycopene didn’t have much antioxidant and antimicrobial activity compared to the standard ascorbic acid and amoxicillin antibiotic, it can be used as a natural antioxidant and antimicrobial drug in periodontics for the treatment of periodontal diseases without any side-effects like the chemical drugs which are used commonly.
Keywords: Antimicrobial, antioxidant, lycopene, nanoparticles, oral pathogens
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