ANTIMICROBIAL AND CYTOTOXIC ACTIVITY OF Adhatoda vasica MEDIATED COPPER NANO PARTICLE
J. DHIVYADHARSHINI
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
LAKSHMINARAYANAN ARIVARASU
Saveetha University, Chennai, India [JD]. Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
S. RAJESH KUMAR
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
*Author to whom correspondence should be addressed.
Abstract
Aim: The aim of the study is to investigate the antimicrobial and cytotoxic effect of Adhatoda vasica mediated copper nanoparticles.
Introduction: Conventional medical drugs have specific targets, but typically also cause significant side effects. Throughout Ayurveda, the conventional form of Indian medicine is commonly referred to as vasaca, which may be a well-known herb in the indigenous drug system. It acts as a sedative, expectorant, antispasmodic, anthelmintic, bronchial antiseptic and bronchodilator.
Materials and Methods: Copper nanoparticles were synthesised separately and then mixed in equal proportion with the prepared plant extract. Centrifugation was done and the pellets were collected separately after the process for further testing. Microorganisms such as S. mutans, Lactobacillus and Candida albicans were chosen and cultured in respective culture media. The Copper Nanoparticles synthesised is introduced into cultured petri plates to observe the changes. For calculating the cytotoxic activity, dividing the estimated number of dead nauplii to the total number and the proportion of motility was calculated.
Results: The zone of inhibition was found to be increasing in both bacterial and fungal species as the concentration of A. vasica CuNP increases. Therefore, A. vasica CuNP was shown to have more effective antimicrobial activity than the standard antibiotics. The cytotoxic effect of A. vasica CuNP shows that as the concentration increases there is a significant increase in the percentage of lethality.
Conclusion: As a result, Adhatoda vasica mediated CuNP expresses good antimicrobial activity and it tends to have a potent cytotoxic activity. Hence this can be taken for further evaluation.
Keywords: Adhatoda vasica, copper nanoparticle, green synthesis, antimicrobial activity, cytotoxic activity