ANTI INFLAMMATORY AND CYTOTOXIC EFFECT OF CLOVE AND CINNAMON HERBAL FORMULATION
T. PRANATI
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai -77, India.
ARVINA RAJASEKAR
Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai -77, India.
S. RAJESHKUMAR
Department of Pharmacology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai -77, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Inflammation is the key finding in oral diseases, such as dental caries, gingivitis, periodontitis and oral mucosal lesions. It is important to devise new topical agents to reduce the inflammation. Plant extracts are widely being used in the pharmaceutical industries as they are naturally available and fewer side effects.
Aim: The aim of this study is to check the anti inflammatory and cytotoxic effect of clove and cinnamon herbal formulation.
Materials and Methods: Clove and cinnamon were powdered. 5 grams of powdered clove and 5 grams of powdered cinnamon were weighed and mixed with 100ml distilled water. Aqueous extract of clove and cinnamon was prepared. The extract was then tested for cytotoxicity by using brined shrimp nauplii. Anti inflammatory activity of the extract was detected using Bovine Serum Albumin.
Results: The aqueous extract of clove and cinnamon extract showed highest absorbance at a concentration of 50 μl (90.3%) when subjected to inhibition of albumin denaturation assay to check for its anti inflammatory activity at a wavelength of 660 nm. The cytotoxicity of the extract was lowest at 10 μl.
Conclusion: The prepared extract of clove and cinnamon proved to be a potent antioxidant and antimicrobial agent and can therefore be used for the application in the medical field. The cytotoxicity assay revealed that the extract can be incorporated in biomedical uses, thereby concluding that clove and cinnamon extract can be a pioneering step towards the shift to greener medicine.
Keywords: Anti inflammatory, brine shrimp method, cytotoxicity, herbal formulation, plant extracts, protein denaturation assay