THE USE OF THREE FUNGAL STRAINS IN PRODUCING OF INDOLE-3-ACETIC ACID AND GIBBERELLLIC ACID
BAKHORA TURAEVA
Institute of the Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan.
AZAM SOLIEV
Republican Scientific and Practical Center of Sports Medicine, Tashkent, Uzbekistan.
FARKHOD ESHBOEV
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan.
LUKHMON KAMOLOV
Karshi State University, Kashkadarya region, Uzbekistan.
NODIRA AZIMOVA
Institute of the Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan.
HUSNIDDIN KARIMOV
Institute of the Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan.
NIGORA ZUKHRITDINOVA
Institute of the Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan.
KHURSHIDA KHAMIDOVA
Institute of the Microbiology, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan.
*Author to whom correspondence should be addressed.
Abstract
In this work, the effective Indole-3-acetic acid (IAA) and Gibberellic acid (GA) producing conditions by Trichoderma harzianum UzCF-55, Penicillium canescens UzCF-54, Fusarium moniliforme UzGC-12 fungal strains have been investigated. It was found that the optimal pH of the nutrients for the strains was 5.5 for Mandel’s and 6.8 for Czapek's mediums, with the incubation temperature intervals of 28-30°C for 10 days. Quantitative analysis of the IAA synthesized by T. harzianum UzCF-55 revealed that the highest amount of the acid was 1,16 mg/mL and 0.74 mg/mL, respectively on the sixth day of the exponential phase of micromycetes growth, while it was 0.318 mg/ml and 0.17 mg/mL for the GA after ninth day. The amounts of IAA and GA synthesized by P. canescens UzCF-54 were 0.98 mg/mL and 0.38 mg/mL in the similar days of incubation, also showing greater amounts than the control strain. F. moniliforme UzGC-12 strain synthesized 0.63 mg/mL IAA and 0.39 mg/mL GA, respectively. High performance liquid chromatography-mass spectrometry (HPLC-MS) analyses of the micromycetes revealed the mass fractions of 174.00 m/z corresponding to the molecular mass of IAA, 363.00 m/z of GA7 and 361.00 m/z of GA3, indicating gibberellins syntheses by strains.
Keywords: T. harzianum, P. canescens, F. moniliforme, fungi, gibberellic acid, indole-3-acetic acid
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