CONTROLLING Streptococcus mutans USING SELENIUM NANOPARTICLES MEDIATED THROUGH Symplocos racemosa
K. SATHVIKA
Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600077, India
S. RAJESHKUMAR *
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600077, India
*Author to whom correspondence should be addressed.
Abstract
Background: Streptococcus mutans (Sm), the harbinger for dental decay is a pathogenic bacterium that is even associated with the pathogenesis of cardiovascular disorders when left unsupervised. However, with proper inhibitory measures, we can curb its role in exaggerating morbidity. Thus, the aim of our study was to examine the plausible antibacterial potential of selenium nanoparticles (Se-NPs) synthesized using Symplocos racemosa (Sr).
Materials and Methods: Sr was pulverised and made into a filtered herbal formulation. Sodium selenite solution was added to it and the conjunction was kept in a magnetic stirrer for the synthesis of nanoparticles. The Se-NPs were characterized using UV-vis-spectroscopy and were centrifuged until the final pellet was collected. A fresh bacterial suspension of Sm was dispersed on a Muller-Hinton Agar containing plate. Se-NP concentrations of (50, 100 & 150) μL were incorporated into the wells and the plate was incubated at 37°C for 24 hours. Antibiotics were used as a control and the zones of inhibition were recorded.
Results: There was an observable colour change after the synthesis of Se-NPs. The prepared particles were then characterised by UV-vis spectroscopy and a peak seen at 280 nm using UV-vis-spectroscopy. The zones of inhibition increased in size with an increase in Se-NP concentration. 150 μL of our Se-NPs was the most effective against Sm closely followed by the antibacterial control.
Conclusion: Se-NPs synthesized using Sr is an effective antibacterial agent against Sm. They are non-toxic, rapid and easy to synthesize and could possibly be an inexpensive solution for controlling Sm.
Keywords: Selenium nanoparticles, Symplocos racemosa, antibacterial, Streptococcus mutans, caries.