PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Background: The plant ginger (Zingiber officinale), Roscoe belongs to Zingiberacea family, may be it is a perennial herb which contain thick and tuberous rhizomes. Plants are utilized in preparation of traditional medicines for many years and those medications were prepared from locally developed wild plants. This study was organized to investigate E. elatior flower’s methanol extract against many disease causing fungi and bacteria and also to investigate antimicrobial sources as well as to report on its antioxidant activities and screaming test to phytochemical.

Methodology: All experiments are done in university of Lahore research laboratories of biotechnology and plant laboratories. Using Ethanol extraction, N-Hexane Extraction, Aqueous Extraction Rotary evaporator. All chemicals were used in this experiment or reagent study analytically and given as; n-hexane, ethanol, whitman filter paper, nutrient agar, SDA (Sabouraud Dextrose Agar) distilled water and nutrient Broth. The prepared extracts were stored in autoclaved 25 ml McCartney bottles in 4°C refrigerator until use. The bottles were carefully and labelled correctly prior to storage.

Results: There were four bacterial strains such as Bacillus cereus, Escherichia coli, Aceromonas, Pseudomonas aeruginosa along with the positive controls the antifungal activity of Zingiber officinale is evaluated against several types of pathogens with different concentration like Aspergillus flavus, Aspergillus flavus and Aspergillus niger. All types of fungus are applied on the culture of different fungus by method of disc diffusion. Firstly, of all we extract the rhizome with positive control of fungus. Rhizome extract show the maximum results in Aspergillus niger and Aspergillus flavus 17 mm and 17.5 mm of zone of inhibition respectively but other types fungi species also gives the good results of zone of inhibition.

Conclusion: The result has shown the dose dependent antimicrobial activity of n-hexane extract which was prepared from rhizome of ginger (Zingiber officinale). There were four bacterial strains such as Bacillus cereus, Escherichia coli, Aceromonas, Pseudomonas aeruginosa along with the positive controls. Pseudomonas aeruginosa, Aceromonas showed the maximum antibacterial activity which is greater beside positive control in Zingiber officinale. On the other hand, the minimum antibacterial ethanol extraction founds against Escherichia coli. Positive control was not showed inhibition zones.