ANTIBACTERIAL, ANTIFUNGAL AND ANTICANCER ACTIVITIES OF MULTI- STRESS AFFECTED Zingiber officinale PLANT EXTRACTS

Main Article Content

HAJRA ZULFIQAR WINNING
QURBAN ALI
MUHAMMAD SAJID RASHID
ARIF MALIK

Abstract

Background: The plant ginger (Zingiber officinale), Roscoe belongs to Zingiberacea family, may be it is a perennial herb which contain thick and tuberous rhizomes. Plants are utilized in preparation of traditional medicines for many years and those medications were prepared from locally developed wild plants. This study was organized to investigate E. elatior flower’s methanol extract against many disease causing fungi and bacteria and also to investigate antimicrobial sources as well as to report on its antioxidant activities and screaming test to phytochemical.

Methodology: All experiments are done in university of Lahore research laboratories of biotechnology and plant laboratories. Using Ethanol extraction, N-Hexane Extraction, Aqueous Extraction Rotary evaporator. All chemicals were used in this experiment or reagent study analytically and given as; n-hexane, ethanol, whitman filter paper, nutrient agar, SDA (Sabouraud Dextrose Agar) distilled water and nutrient Broth. The prepared extracts were stored in autoclaved 25 ml McCartney bottles in 4°C refrigerator until use. The bottles were carefully and labelled correctly prior to storage.

Results: There were four bacterial strains such as Bacillus cereus, Escherichia coli, Aceromonas, Pseudomonas aeruginosa along with the positive controls the antifungal activity of Zingiber officinale is evaluated against several types of pathogens with different concentration like Aspergillus flavus, Aspergillus flavus and Aspergillus niger. All types of fungus are applied on the culture of different fungus by method of disc diffusion. Firstly, of all we extract the rhizome with positive control of fungus. Rhizome extract show the maximum results in Aspergillus niger and Aspergillus flavus 17 mm and 17.5 mm of zone of inhibition respectively but other types fungi species also gives the good results of zone of inhibition.

Conclusion: The result has shown the dose dependent antimicrobial activity of n-hexane extract which was prepared from rhizome of ginger (Zingiber officinale). There were four bacterial strains such as Bacillus cereus, Escherichia coli, Aceromonas, Pseudomonas aeruginosa along with the positive controls. Pseudomonas aeruginosa, Aceromonas showed the maximum antibacterial activity which is greater beside positive control in Zingiber officinale. On the other hand, the minimum antibacterial ethanol extraction founds against Escherichia coli. Positive control was not showed inhibition zones.

Keywords:
Antibacterial, anticancer, antifungal, Zingiber officinale.

Article Details

How to Cite
WINNING, H. Z., ALI, Q., RASHID, M. S., & MALIK, A. (2020). ANTIBACTERIAL, ANTIFUNGAL AND ANTICANCER ACTIVITIES OF MULTI- STRESS AFFECTED Zingiber officinale PLANT EXTRACTS. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(53-54), 106-117. Retrieved from https://ikprress.org/index.php/PCBMB/article/view/5583
Section
Original Research Article

References

Matan N, Rimkeeree H, Mawson AJ, Chompreeda P, Haruthaithanasan V, Parker M. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. Intern J Food Microbiol. 2006;107(2):180-5.

Norajit K, Laohakunjit N, Kerdchoechuen O. Antibacterial effect of five Zingiberaceae essential oils. Molecules. 2007;12(8):2047-2060.

Gomaa NF, Hashish MH. The inhibitory effect of garlic (Allium sativum) on growth of some microorganisms. The Journal of the Egyptian Public Health Association. 2003;78(5-6):361-72.

Avato P, Tursil E, Vitali C, Miccolis V, Caddido V. Allyl sulphide constituents of garlic volatile oil as antimicrobial agents. Phytomedicine. 2000;7:239-243.

McGee H. On food and cooking. The science and lore of the kitchen. 2nd Edition. Harold McGee (Ed). New York. 2004;425-426.

O’Hara M, Keifer D, Farrel K, Kemper K. A review of 12 commonly used medicinal herbs. Archives. Fam. Med. 1998;7:523-536.

Danish P, Ali Q, Hafeez MM, Malik A. Antifungal and antibacterial activity of Aloe vera plant extract. Biol Clin Sci Res J. 2020;e003.

Ali BH, Blunden G, Tanira MO, Nemmar A. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): A review of recent research. Food Chem Toxicol. 2008;6(2):409-420.

Panpatil VV, Tattari S, Kota N, Nimgulkar C, Polasa K. In vitro evaluation on antioxidant and antimicrobial activity of spice extracts of ginger, turmeric and garlic. J Pharmacognosy and Phytochemistry. 2013;2(3):143-148.

Muhammad HF, Ahmad A, Tahir M. Response of different phosphorus levels and application methods on the growth and quality of linseed crop. Biol Clin Sci Res J. 2020;e019.

Boer ER, Ward NJ, Manser MP, Yamamura T, Kuge N. Driver performance assessment with a car following model; 2005.

Habib SN, Makpol HM, Hamid S, NAA, Das S, Ngah WZW, Yusuf YAM. Ginger extract (Zingiber officinale) has anti-cancer and anti-inflammatory effects on ethionine-induced hepatoma rats. Clinics. 2008;63(6):807-813.

Khalil R, Ali Q, Hafeez MM, Malik A. Phenolic acid profiling by RP-HPLC: Evaluation of antibacterial and anticancer activities of Conocarpus erectus plant extracts. Biol Clin Sci Res J. 2020; e010.

Srivastava KC, Mustafa T. Ginger (Zingiber officinale) in rheumatism and musculoskeletal disorders. Med Hypotheses. 1992;39:342-348.

Ali Q, Khalil R, Nadeem M, Azhar MM, Hafeez MM, Malik A. Antibacterial, antioxidant activities and association among plant growth related traits of Lepidium draba. Biol Clin Sci Res J. 2020;e011.

Gull IM, Saeed H, Shaukat SM, Aslam ZQ, Samra, Athar AM. Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Ann Clin Microbiol Antimicrob. 2012;11:8. DOI: 10.1186:1476-0711-11-8

Malu AO, Okeke EN, Ladep NG, Agaba EI. Hepatitis B vaccination status and needle stick injuries among medical students in a Nigerian university. Niger J. Med. 2008;17(3):330-2.

Sykes SR. The inheritance of salt exclusion in woody perennial fruit species. Plant and Soil. 1992;197:123-129.

Al-Amin ZM, Thomson M, Al-Qattan KK, Peltonen-Shalaby R, Ali M. Anti-diabetic and hypolipidaemic properties of ginger (Zingiber officinale) in streptozotocin-induced diabetic rats. British J. Nutrition. 2006;96(4):660-666.

Afshari AT, Shirpoor A, Farshid R, Saadatian, Rasmi Y, Saboory E, Allameh A. The effect of ginger on diabetic nephropathy, plasma antioxidant capacity and lipid peroxidation in rats. Food Chemistry. 2007;101(1):148-153.

Ahmad S, Hera Z, Hanif MS, Syed AH. Effects of carbosulfan on the biology of bird cherry oat aphid. Biol Clin Sci Res J. 2020;e014.

Melvin MJ, Jayachitra J, Vijayapriya M. Antimicrobial activity of some common spices against certain human pathogens. J Medicinal Plants Res. 2009;3(11):1134-1136.

Rajalakshmi S, Leelavathi R. Dodonaea viscosa (L.) leaves extract as acid corrosion inhibitor for mild steel–a green approach. J Materials Environ Sci. 2013;4(5):625-638.

Poeloengan M. The effect of red ginger (Zingiber officinale Roscoe) extract on the growth of mastitis causing bacterial isolates. African Journal of Microbiology Research. 2011;5(4):382-8.

Belguith-Hadriche O, Bouaziz Jamoussi MK, El Feki A, Sayadi SF, Makni-Ayedi. Lipid-lowering and antioxidant effects of an ethyl acetate extract of fenugreek seeds in high-cholesterol-fed rats. Journal of Agricultural and Food Chemistry. 2010;58(4):2116-22.

Omoya FO. Antifungal activities of a pasture honey and ginger (Zingiber officinale) extracts on some pathogenic fungi. J Sci Technol (Ghana). 2012;32(2):20-25.

Pattaratanawadee E, Rachtanapun C, Wanchaitanawong P, Mahakarnchanakul W. Antimicrobial activity of spice extracts against pathogenic and spoilage microorganisms. Agric Natural Resour. 2006;40(5):159-65.

Weil A. Antimicrobial activity of ginger against different microorganisms: New York. 2005;300-308.

Yaqoob S, Fatima N, Khan S, Ali Q, Hafeez MM, Malik A. Begomoviruses and betasatellites associated with CLCuD. Biol Clin Sci Res J. 2020;e002.

Ernst E, Pittler MH. Efficacy of ginger for nausea and vomiting: A systematic review of randomized clinical trials. British Journal of Anesthesia. 2000;84(3):367–371.

Lee WJ, Lucey JA. Structure and physical properties of yogurt gels: Effect of inoculation rate and incubation temperature. J Dairy Sci. 2004;87(10):3153-64.

Yin PG, Sui EB, Affar Y, Shi C, Brignone NR, Wall M, Donohoe MP, Luke D, Calvo SR, Grossman, Shi Y. Yin Yang 1 is a negative regulator of p53. Cell. 2004;117(7):859-72.

Yusha’u M, Garba L, Shamsuddeen U. In vitro inhibitory activity of garlic and ginger extracts on some respiratory tract isolates of gram-negative organisms. International Journal of Biomedical and Health Sciences. 2008;4(2).

Naseem S, Ali Q, Malik A. Evaluation of maize seedling traits under salt stress. Biol Clin Sci Res J. 2020;e025.

Muqadas S, Ali Q, Malik A. Genetic association among seedling traits of Zea mays under multi stress of salt, heavy metals and drought. Biol Clin Sci Res J. 2020;e026.

Hameed B, Ali Q, Hafeez MM, Malik A. Antibacterial and antifungal activity of fruit, seed and root extracts of Citrullus colocynthis plant. Biol Clin Sci Res J. 2020;e033.

Siddique A, Fateh A, Idrees N, Hafeez MM, Ali Q, Malik A. The epidemics of COVID-19. Biol Clin Sci Res J. 2020;e030.

Anwar Y. Saat menuai kejahatan: sebuah pendekatan sosiokultural kriminologi, hukum, dan HAM. Refika Aditama; 2009.

Lachumy SJ, Sasidharan TS, Sumathy V, Zuraini Z. Pharmacological activity, phytochemical analysis and toxicity of methanol extract of Etlingera elatior (torch ginger) flowers. Asian Pacific J Tropical Medicine. 2010;3(10):769- 774.

Mamun-Or-Rashid AN, Shohel MM, Nayeem MT, Monokesh KS. Compendium ehtnopharmaceutical review on Euphorbia hirta. Ayurpharm Int J Ayur Alli Sci. 2013;2(2):14-21.