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Aim and Background: One of the amino acids that has a benzene ring as a side chain is Tyrosine. This makes tyrosine an aromatic amino acid that has the ability to absorb UV. However, this absorption can cause degradation of tyrosine. Antioxidant compounds have the potential to be used as photoprotectors that can prevent photodegradation. Kersen fruit (Muntingia calabura L) has been reported to show high antioxidant activity due to its high flavonoid content, in the glucosylated and free forms. This study aims to determine the inhibition of tyrosine photodegradation activity by lyophilisate, extracts and fractions of Kersen fruit (Muntingia calabura L.) so that the kersen fruits can be developed as photo-protective agent.
Materials and Methods: Kersen fruit (Muntingia calabura L.) lyophilized using a freeze dryer to obtain the lyophilisate of Kersen fruit. The extraction method used maceration with ethanol 70% Fractination used n-hexane, ethyl acetate and ethanol as solvents. The Inhibition value of Lyophilisate, extract and fractions of Kersen fruit was determined by measuring the remaining tyrosine after irradiation and ketoprophen addition then compared to the amount remaining tyrosine from ascorbic acid.
Results and Discussion: lyophilisate, ethanol extract, n-hexane fraction, ethyl acetate fraction and ethanol fraction of kersen fruit (Muntingia calabura L.) has inhibitory activity, among others: 0.042; 0.333; 0.354; 0.931; and 0.328 times compared with vitamin C at the same concentration.
Conclusion: The conclusion of this research it can be concluded the ethyl acetate fraction showed the most relate the inhibition value to ascorbic acid against the tyrosine photodegradation.
Schöneich C. Photo-degradation of therapeutic proteins: Mechanistic aspects. Pharm. Res. 2020;37(3):45.
Irianti T, Fakhrudin N, Efendi Hartomo S, Astuti SPY, Ksumaningtyas RA, et al. Comparation of several plants extract and vitamin c inhibition activity to tyrosine photodegradation induced by ketoprofen and its total phenolic compounds. Trad. Med. J; 2016.
Pangemanan DA, Suryanto E, Yamlean PVY. Phytochemical screening, antioxidant activity and sunscreen activity of corn (Zea Mays L.). Pharmacon; 2020.
Irianti T, Sulaiman, Syaifullah TN, Fakhrudin N, Astuti S, Testikawati N, Faridah S, Khasanah. Preparation of sunscreen preparations ethanol extract of mahkota dewa fruit (Phaleria macrocarpa), Tyrosine Photodegradation Inhibition Activity and Total Phenolic Content. Maj. Farm; 2019.
Cuadro Mogollon OF, Gonzalez-Cuello RE, Gonzalez Lopez JS. In vitro antibacterial and antioxidant activity of muntingia calabura fruits extract. Contemp. Eng. Sci.; 2018.
Preethi K, Premasudha P, Keerthana K. Anti-inflammatory activity of Muntingia calabura fruits. Pharmacogn. J.; 2012.
Pereira GA, Arruda HS, de Morais DR, Eberlin MN, Pastore GM. Carbohydrates, volatile and phenolic compounds composition, and antioxidant activity of calabura (Muntingia calabura L.) fruit. Food Res. Int; 2018.
Harborne JB. Phytochemical methods a guide to modern tecniques of plant analysis, third edition. Chapman Hall; 1998.
Iyer D, Sharma BK, Patil UK. Isolation of bioactive phytoconstituent from Alpinia galanga L. with anti-hyperlipidemic activity. J. Diet. Suppl; 2013.
Chen ML, Yang DJ, Liu SC. Effects of drying temperature on the flavonoid, phenolic acid and antioxidative capacities of the methanol extract of citrus fruit (Citrus sinensis (L.) Osbeck) peels. Int. J. Food Sci. Technol; 2011.
Molina-Calle M, Priego-Capote F, Luque De Castro MD. Development and application of a quantitative method for determination of flavonoids in orange peel: Influence of sample pretreatment on composition. Talanta; 2015.
Mandal SC, Mandal V, Das AK. Essentials of botanical extraction: Principles and applications; 2015.
Šavikin K et al. Activity guided fractionation of pomegranate extract and its antioxidant, antidiabetic and antineurodegenerative properties. Ind. Crops Prod; 2018.
Bandiola TMB. Extraction and Qualitative phytochemical screening of medicinal plants: A brief summary. Int. J. Pharm; 2013.
Teresa M, Petersen S, Prakash G. UV light effects on proteins: From photochemistry to nanomedicine. In Molecular Photochemistry - Various Aspects; 2012.
Triyono A. studying the effect of some acid addition on protein isolation process of green nut isolate protein flour (Phaseolus radiatus L.). In Seminar Rekayasa Kimia dan Proses; 2010.
Spielmann H. et al. The international EU/COLIPA in vitro phototoxicity validation study: Results of phase II (blind trial). Part 1: The 3T3 NRU phototoxicity test,” Toxicol. Vitr; 1998.
Hwang JS. et al. Effects of freeze-drying on antioxidant and anticholinesterase activities in various cultivars of kiwifruit (Actinidia spp.). Food Sci. Biotechnol; 2017.
Oprica L. Antohe RG, Verdes A, Grigore MN. Effect of freeze-drying and oven-drying methods on flavonoids content in two romanian grape varieties. Rev. Chim.; 2019.
Chang TC, Chang ST. Wood photostabilization roles of the condensed tannins and flavonoids from the EtOAc fraction in the heartwood extract of Acacia confusa. Wood Sci. Technol; 2018.
Sujono TA, Dian Kusumowati IT, Munawaroh R. Immunomodulatory activity of Muntingia calabura L. fruits using carbon clearance assay and their total flavonoid and phenolic contents. Asian J. Pharm. Clin. Res; 2019.
Cruces E, Flores-Molina MR, Díaz MJ, Huovinen P, Gómez I. Phenolics as photoprotective mechanism against combined action of UV radiation and temperature in the red alga Gracilaria chilensis?. J. Appl. Phycol. 2018.