ESTIMATION OF WEDELOLACTONE IN Eclipta prostrata (L.) L BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AFTER MICROBIAL TREATMENTS AT TWO LEVELS OF WATER AVAILABILITY
SHILPAM SINHA *
Department of Botany, M.M.V., Banaras Hindu University, Varanasi-221005, UP, India
RICHA RAGHUWANSHI
Department of Botany, M.M.V., Banaras Hindu University, Varanasi-221005, UP, India
*Author to whom correspondence should be addressed.
Abstract
Wedelolactone level in methanolic extract of E. prostrata undergone different microbial treatments comprised 1. Bacillus megaterium (B), 2. Trichoderma harzianum (T), 3. Funneliformis mosseae (F) and 4. consortia of F.mosseaeand T. harzianum (FT), 5. B. megaterium and T. harzianum (BT), 6. F. mosseae and B. megaterium (FB) and 7. Control exposed to irrigated and drought soil conditions were quantified by reverse-phase high performance liquid chromatography-photodiode array (RP-HPLC) method. The above method shows reproducible, high resolution, accurate and economical results. The wedelolactone level in E. prostrata was found higher after treatment with microbial consortia of Funelliformis mosseae and Bacillus megaterium (FB) under both drought and irrigated soil conditions. Drought condition enhanced the wedelolactone concentration in plants compared to irrigated soil condition. A maximum amount of wedelolactone was found after treatment of FB under drought stress.
Keywords: Eclipta prostrata, drought stress, high performance liquid chromatography, wedelolactone, microbial treatments.