EFFECT OF AUXINS (IAA, IBA AND NAA) ON CLONAL PROPAGATION OF Solanum procumbens STEM CUTTINGS
L. H. TIEN
Northern Central Center of Medicinal Materials Research, National Institute of Medicinal Materials, Thanh Hoa, Vietnam.
L. D. CHAC
Faculty of Natural Sciences, Hong Duc University, Thanh Hoa, Vietnam.
L. T. L. OANH
Thanh Hoa Medical College, Thanh Hoa, Vietnam.
P. T. LY
Northern Central Center of Medicinal Materials Research, National Institute of Medicinal Materials, Thanh Hoa, Vietnam.
H. T. SAU
Northern Central Center of Medicinal Materials Research, National Institute of Medicinal Materials, Thanh Hoa, Vietnam.
N. HUNG
Vietnam-Hungary Industrial University, Hanoi, Vietnam.
V. Q. THANH
Vietnam-Russia Tropical Center, Hanoi, Vietnam.
R. V. DOUDKIN
Botanical Garden-Institute, Far-East Branch, Russian Academy of Sciences, Vladivostok, Russia and School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia
B. B. THINH *
School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia.
*Author to whom correspondence should be addressed.
Abstract
Solanum procumbens Lour. is a precious medicinal plant with plenty applications in medicine and pharmacy. Auxin is a plant hormone which is critical to the induction and growth of roots of cuttings. In this study, an experiment was conducted to evaluate the effects of indole acetic acid (IAA), indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) on the rooting and sprouting of S. procumbens stem cuttings. The experiment involved four distinctive concentrations of IAA (500, 1000, 1500 and 2000 ppm), IBA (500, 1000, 1500 and 2000 ppm) and IBA + NAA (250 + 250, 500 + 500, 750 + 750 and 1000 + 1000 ppm) along with the control group used to treat S. procumbens cuttings. The experiment applied the randomized complete block (RCB) method with 3 replications. The results showed that IAA, IBA and NAA had a remarkable influence on rooting and sprouting abilities of S. procumbens cuttings. The best records of sprouting rate (92.34%), root number (32.25), root length (6.8 cm), root weight (0.574 g), sprout length (11.7 cm) and leaf-pair number (7.5) were obtained in cuttings treated with IBA at 500 ppm. Furthermore, the beneficial effects of auxins wore off as their concentrations increased. In general, this study emphasizes that IBA at 500 ppm is the most effective auxin in terms of stimulating rooting and sprouting in S. procumbens cuttings. The results of the study also promise to create a supply of high-quality S. procumbens seedlings for gardeners.
Keywords: Solanum procumbens, auxin, propagation, stem cuttings, rooting, sprouting, seedling