ANTIMICROBIAL ACTIVITY OF CLOVE AND CINNAMON MEDIATED SILVER NANOPARTICLES AGAINST Lactobacillus SPECIES - AN in vitro STUDY

Main Article Content

SHREYA KOTHARI
ARVINA RAJASEKAR
S. RAJESHKUMAR

Abstract

Background: Clove and cinnamon is also known for a number of medicinal biological activities, such as antibacterial, antifungal, insecticidal and antioxidant properties, and is used traditionally as a savoring agent and antimicrobial material in food. Lactobacilli are known contributing to the balance of microflora and the primary cause for dental caries. Formulations made of herbal products have not been put to use in abundance in comparison to synthetic drugs which pose side effects.

Aim: The aim of this study was to assess and evaluate the antimicrobial activity of clove-cinnamon mediated silver nanoparticles against Lactobacilli.

Materials and Methods: The clove-cinnamon powder procured for the aqueous extract solution was boiled, filtered and added with silver nitrate solution for nanoparticles synthesis and then centrifuged at 8000 rpm to form pellets. The solutions at different concentrations were added to the wells of the inoculated agar plate and left for incubation. The zones of inhibition for every concentration were then measured using a scale by the agar diffusion method.

Results: The antimicrobial activity of silver nanoparticles with clove and cinnamon extracts against Lactobacilli was most effective at the highest concentration (15 mm). It was found to be more than Amoxicillin (8 mm).

Conclusion: The study proved that clove and cinnamon extract mediated silver nanoparticles showed good antimicrobial activity. Further research and clinical trials needs to be done in future to make new formulations.

Keywords:
Clove, cinnamon, nanoparticles, antimicrobial, lactobacilli, agar diffusion.

Article Details

How to Cite
KOTHARI, S., RAJASEKAR, A., & RAJESHKUMAR, S. (2020). ANTIMICROBIAL ACTIVITY OF CLOVE AND CINNAMON MEDIATED SILVER NANOPARTICLES AGAINST Lactobacillus SPECIES - AN in vitro STUDY. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 21(59-60), 1-7. Retrieved from https://ikprress.org/index.php/PCBMB/article/view/5617
Section
Original Research Article

References

Swamy M, Sinniah U. A comprehensive review on the phytochemical constituents and pharmacological activities of Pogostemon cablin Benth.: An aromatic medicinal plant of industrial importance. Molecules. 2015;20:8521–8547.

Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 1999;12:564–582.

Burt S. Essential oils: Their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology. 2004;94:223–253.

Dorman HJD, Deans SG. Antimicrobial agents from plants: Antibacterial activity of plant volatile oils. Journal of Applied Microbiology. 2000;88:308–316.

Matan N, Rimkeeree H, Mawson AJ, et al. Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions. Int J Food Microbiol. 2006;107:180–185.

EBSCOhost – 108326333. Antifungal activity of ginger and cinnamon leaf essential oils on mango anthracnose disease causing fungi (C. gloeosporioides).

Pereira-Cenci T, Cenci MS, Fedorowicz Z, et al. Antibacterial agents in composite restorations for the prevention of dental caries. Cochrane Database Syst Rev. 2009;CD007819.

Martinez-Gutierrez F, Olive PL, Banuelos A, et al. Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. Nanomedicine: Nanotechnology, Biology and Medicine. 2010;6:681–688.

Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria. Journal of Colloid and Interface Science. 2004;275:177–182.

Maheshwari M, Ahmad I, Althubiani AS. Multidrug resistance and transferability of bla CTX-M among extended-spectrum β-lactamase-producing enteric bacteria in biofilm. Journal of Global Antimicrobial Resistance. 2016;6:142–149.

Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances. 2009;27:76–83.

Rai M, Kon K, Ingle A, et al. Broad-spectrum bioactivities of silver nanoparticles: The emerging trends and future prospects. Appl Microbiol Biotechnol. 2014;98:1951–1961.

Lara HH, Ayala-Núñez NV, del Carmen Ixtepan Turrent L, et al. Bactericidal effect of silver nanoparticles against multidrug-resistant bacteria. World Journal of Microbiology and Biotechnology. 2010;26:615–621.

Banerjee M, Mallick S, Paul A, Chattopadhyay A, Ghosh SS. Heightened reactive oxygen species generation in the antimicrobial activity of a three component iodinated chitosan− silver nanoparticle composite. Langmuir. 2010;26(8):5901- 8.

Mohanpuria P, Rana NK, Yadav SK. Biosynthesis of nanoparticles: technological concepts and future applications. Journal of Nanoparticle Research. 2008;10:507–517.

Dipankar C, Murugan S. The green synthesis, characterization and evaluation of the biological activities of silver nanoparticles synthesized from Iresine herbstii leaf aqueous extracts. Colloids and Surfaces B: Biointerfaces. 2012;98:112–119.

Ezhilarasan D, Apoorva VS, Ashok Vardhan N. Syzygium cumini extract induced reactive oxygen species-mediated apoptosis in human oral squamous carcinoma cells. J Oral Pathol Med. 2019;48:115–121.

Gajendran PL, Parthasarathy H, Tadepalli A. Comparative evaluation of cathepsin K levels in gingival crevicular fluid among smoking and nonsmoking patients with chronic periodontitis. Indian J Dent Res. 2018;29:588–593.

Kaarthikeyan G, Jayakumar ND, Sivakumar D. Comparative evaluation of bone formation between PRF and blood clot alone as the sole sinus-filling material in maxillary sinus augmentation with the implant as a tent pole: A randomized split-mouth study. J Long Term Eff Med Implants. 2019;29:105–111.

Arjunkumar R. Nanomaterials for the management of periodontal diseases. In: Chaughule RS (Ed) Dental Applications of Nanotechnology. Cham: Springer International Publishing. 2018;203–215.

Ravi S, Malaiappan S, Varghese S, et al. Additive effect of plasma rich in growth factors with guided tissue regeneration in treatment of intrabony defects in patients with chronic periodontitis: A split-mouth randomized controlled clinical trial. J Periodontol. 2017;88:839–845.

Kavarthapu A, Malaiappan S. Comparative evaluation of demineralized bone matrix and type II collagen membrane versus eggshell powder as a graft material and membrane in rat model. Indian J Dent Res. 2019;30:877–880.

Murthykumar K, Arjunkumar R, Jayaseelan VP. Association of vitamin D receptor gene polymorphism (rs10735810) and chronic periodontitis. J Investig Clin Dent. 2019;10:e12440.

Ramesh A, Vellayappan R, Ravi S, et al. Esthetic lip repositioning: A cosmetic approach for correction of gummy smile - A case series. J Indian Soc Periodontol. 2019;23:290–294.

Ramesh A, Varghese S, Jayakumar ND, et al. Comparative estimation of sulfiredoxin levels between chronic periodontitis and healthy patients - A case-control study. J Periodontol. 2018;89:1241–1248.

Kavarthapu A, Thamaraiselvan M. Assessing the variation in course and position of inferior alveolar nerve among South Indian population: A cone beam computed tomographic study. Indian J Dent Res. 2018;29:405–409.

Ramesh A, Ravi S, Kaarthikeyan G. Comprehensive rehabilitation using dental implants in generalized aggressive periodontitis. J Indian Soc Periodontol. 2017;21:160–163.

Jain M, Nazar N. Comparative evaluation of the efficacy of intraligamentary and supraperiosteal injections in the extraction of maxillary teeth: A randomized controlled clinical trial. J Contemp Dent Pract. 2018;19:1117–1121.

Vijayashree Priyadharsini J. In silico validation of the non-antibiotic drugs acetaminophen and ibuprofen as antibacterial agents against red complex pathogens. J Periodontol. 2019;90:1441–1448.

Rk S, Sanjukta RK. Green synthesis of silver nanoparticles using plants. International Journal of Nanomedicine and Nanosurgery (ISSN 2470-3206); 2. Epub Ahead of Print 2016.

DOI: 10.16966/2470-3206.110

Agarwal H, Venkat Kumar S, Rajeshkumar S. A review on green synthesis of zinc oxide nanoparticles – An eco-friendly approach. Resource-Efficient Technologies. 2017;3:406–413.

Mechanism of plant-mediated synthesis of silver nanoparticles – A review on biomolecules involved, characterisation and antibacterial activity. Chem Biol Interact. 2017;273:219–227.

Phyto-assisted synthesis, characterization and applications of gold nanoparticles – A review. Biochemistry and Biophysics Reports. 2017;11:46–57.

Menon S, Rajeshkumar S, Kumar V. A review on biogenic synthesis of gold nanoparticles, characterization, and its applications. Resource-Efficient Technologies. 2017;3(4):516-27.

Synthesis and biomedical applications of cerium oxide nanoparticles – A review. Biotechnology Reports. 2018;17:1–5.

Happy Agarwal, Soumya Menon, Venkat Kumar S, et al. Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route. Chem Biol Interact. 2018;286:60– 70.

Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism. Colloids Surf B Biointerfaces. 2018;170:280–292.

Sintubin L, Verstraete W, Boon N. Biologically produced nanosilver: Current state and future perspectives. Biotechnology and Bioengineering. 2012;109:2422–2436.

Neu HC. Antimicrobial activity and human pharmacology of amoxicillin. Journal of Infectious Diseases. 1974;129:S123–S131.

Kim JS, Kuk E, Yu KN, et al. Antimicrobial effects of silver nanoparticles. Nanomedicine: Nano-technology, Biology and Medicine. 2007;3:95–101.

Ouwehand AC, Tiihonen K, Kettunen H, et al. In vitro effects of essential oils on potential pathogens and beneficial members of the normal microbiota. Veterinární Medicína. 2010;55:71–78.

Skiba M, Vorobyova V, Pivovarov A, Shakun A, Gnatko E, Trus I. “Green” synthesis of nanoparticles of precious metals: antimicrobial and catalytic properties. Eastern-European Journal of Enterprise Technologies. 2018;5(6):51- 8.