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Background: Sorbitol, also less commonly known as glucitol, is a sugar alcohol with a sweet taste metabolizesdslowly in human body. It is also called the sorbitol-aldose reductase pathway, the polyol pathway appears to be implicated in diabetic complications, especially in microvascular damage to the retina, kidney, and nerves. (Punicaceae-family; Punica granatum-botanical name). Pomegranate juice significantly decreases lipid peroxidation in patients with type 2 diabetes, and systolic blood pressure and serum angiotensin converting enzyme activity in hypertensive patients.
Aim: To evaluate the sorbitol accumulation inhibitory activity of pomegranate extract (Punica granatum).
Materials and Methods: Plant materials-Punica granatum extract used in the study was obtained from Green Chem Herbal extracts and formulations, Bengaluru, India.
The method for extracting the above plant material was according to the methodology by Kesavanarayanan et al. 2013.
Results: Punica granatum extract has markedly inhibited the sorbitol formation with an IC50 of 48.51μg/mL relative to standard ascorbic acid which showed IC50 of 37.77µg/ml.
Conclusions: It is concluded that pomegranate extract is effective in inhibitory action of sorbitol accumulation in the body.
Puneeth HR, Chandra SSP. A review on potential therapeutic properties of Pomegranate (Punica granatum L.). Plant Science Today. 2020;7:9.
Jinu KV, Archana R, Kumar SS, et al. A comprehensive review on neuroprotective effects of pomegranate. International Journal of Research in Ayurveda & Pharmacy. 2016;7:48–51.
Newman RA, Lansky EP, Block ML. Pomegranate: The most medicinal fruit. Basic Health Publications, Inc.; 2007.
Al-Ashaal H, Aboutabl M, Maklad Y, et al. Tropane alkaloids of Atropa belladonna L.: In vitro production and pharmacological profile. Egyptian Pharmaceutical Journal. 2013;12:130.
Ihnat MA, Thorpe JE, Kamat CD, et al. Reactive oxygen species mediate a cellular ‘memory’ of high glucose stress signalling. Diabetologia. 2007;50:1523–1531.
Takahashi-Abbe S, Abbe K, Takahashi N, et al. Inhibitory effect of sorbitol on sugar metabolism of Streptococcus mutans In vitro and on acid production in dental plaque In vivo. Oral Microbiology and Immunology. 2001;16: 94–99.
Keerthiga N, Anitha R, Rajeshkumar RS, et al. Antioxidant activity of cumin oil mediated silver nanoparticles. Pharmacognosy Journal. 2019;11:787–789.
Suhasini SJ, Jennifer Suhasini S, Roy A, et al. The cytotoxic effect of Caralluma fimbriata on KB cell lines. Research Journal of Pharmacy and Technology. 2019;12:4995.
Kandhan TS, Roy A, Lakshmi T, et al. Green synthesis of rosemary oleoresin mediated silver nanoparticles and its effect on oral pathogens. Research Journal of Pharmacy and Technology. 2019;12: 5379.
Reddy JM, Meghana Reddy J, Anitha R, et al. Characterisation of cumin oil mediated silver nanoparticles using UV-visible spectrophotometer and TEM. Research Journal of Pharmacy and Technology. 2019;12:4931.
Vignesh S, Anitha R, Rajesh Kumar S, et al. Evaluation of the antimicrobial activity of cumin oil mediated silver nanoparticles on oral microbes. Research Journal of Pharmacy and Technology. 2019;12:3709.
Pranati T, Anitha R, Rajeshkumar S, et al. Preparation of silver nanoparticles using nutmeg oleoresin and its antimicrobial activity against oral pathogens. Research Journal of Pharmacy and Technology. 2019;12:2799.
Jain A, Anitha R, Rajeshkumar S. Anti inflammatory activity of Silver nanoparticles synthesised using cumin oil. Research Journal of Pharmacy and Technology. 2019;12:2790.
Happy A, Soumya M, Venkat Kumar S, et al. Phyto-assisted synthesis of zinc oxide nanoparticles using Cassia alata and its antibacterial activity against Escherichia coli. Biochemistry and Biophysics Reports. 2019;17:208–211.
Thangavelu L, Balusamy SR, Shanmugam R, et al. Evaluation of the sub-acute toxicity of Acacia catechu willd seed extract in a wistar albino rat model. Regulatory Toxicology and Pharmacology. 2020;113: 104640.
Deepak VN, Lakshminarayanan A, Roy A, et al. Activity of coconut oil mediated effervescent granules as a denture cleanser against C.albicans, S.mutans and E.faecalis. Indian Journal of Public Health Research & Development. 2019;10: 3701.
NS, Swathi N, Sandhiya D, et al. Green synthesis of titanium dioxide nanoparticles using Cassia fistula and its antibacterial activity. International Journal of Research in Pharmaceutical Sciences. 2019;10:856–860.
Rajeshkumar S, Agarwal H, Kumar SV, et al. One-pot synthesis of zinc oxide nanoparticles using orange peel extract and its potential anti-bacterial activity. International Journal of Pharmaceutical Research. 2018;10:574–578.
SB, Balamithra S, Rajeshkumar S, et al. Antibacterial activity of selenium nanoparticles synthesized using Maranta arundinaecea root extract. International Journal of Research in Pharmaceutical Sciences. 2020;11:2695–2700.
Agrawa R, Maheshwari R, Balaraman R, et al. Anti-hyperglycemic and anti-lipidemic activities of diabac (a polyherbal formulation) in streptozotocin- nicotinamide induced type 2 diabetic rats. Pharmacognosy Journal. 2015;7:283–288.
Opara LU, Al-Ani MR, Al-Shuaibi YS. Physico-chemical properties, Vitamin C content, and antimicrobial properties of pomegranate fruit (Punica granatum L.). Food and Bioprocess Technology. 2009;2: 315–321.
Bapodara M, Nagani K, Chanda S. Pharmacognostic and Physicochemical study of Punica granatum L. leaf. Pharmacognosy Journal. 2011;3:29–32.
Rambhade A, Singh S, Rambhade SK, et al. Occurrence, complications, and interventions of diabetes: A new understanding of an old problem. Systematic Reviews in Pharmacy. 2011;2: 8.
Brownlee M. The Pathobiology of diabetic complications: A unifying mechanism. Diabetes. 2005;54:1615–1625.
Chung SSM. Contribution of polyol pathway to diabetes-induced oxidative stress. Journal of the American Society of Nephrology. 2003;14:233–236.
Karasu Ç, Cumaoğlu A, Gúrpinar A, et al. Aldose reductase inhibitory activity and antioxidant capacity of pomegranate extracts. Interdisciplinary Toxicology. 2012;5:15–20.
Kim J, Kim CS, Sohn E, et al. Litsea japonica extract inhibits aldose reductase activity and hyperglycemia-induced lenticular sorbitol accumulation in db/db mice. Evidence-Based Complementary and Alternative Medicine. 2015;1–5.