STIMULATION OF AMYLASE ACTIVITY BY UV EXPOSURE AND POTATO PEELS
HAMSSA EMAD ABDUL- WAHED *
Applied Science-Biotechnology Branch, University of Technology, Baghdad, Iraq
WASNAA HATIF MOHAMMED
Applied Science-Biotechnology Branch, University of Technology, Baghdad, Iraq
MAYSAA ADIL ALI
Applied Science-Biotechnology Branch, University of Technology, Baghdad, Iraq
RANA SHAMIL NOORI
Applied Science-Biotechnology Branch, University of Technology, Baghdad, Iraq
*Author to whom correspondence should be addressed.
Abstract
After isolation, identifying and screening of Amylolytic bacteria from soil, we obtained Bacillus cereus as positive strain. The different effects of pH and potato peels concentrations on Amylase production were studied. As a result pH 7, 15% peels concentration were the best condition for Amylase activity. The effect of UV radiation exposure at different time (0, 2, 4, 6, 8 and 10 minutes) was used to mutate Bacillus cereus., the results revealed that there is an increasing in amylase activity of Bacillus cereus after exposure to UV, while there is a decreasing in Bacillus cereus growth. Determination of amylase activity, depend on assay enzyme extract against standard curve of maltose.
Keywords: Bacillus cereus, amylolytic bacteria, amylase activity.