Main Article Content
After isolation, identifying and screening of Amylolytic bacteria from soil, we obtained Bacillus cereus as positive strain. The different effects of pH and potato peels concentrations on Amylase production were studied. As a result pH 7, 15% peels concentration were the best condition for Amylase activity. The effect of UV radiation exposure at different time (0, 2, 4, 6, 8 and 10 minutes) was used to mutate Bacillus cereus., the results revealed that there is an increasing in amylase activity of Bacillus cereus after exposure to UV, while there is a decreasing in Bacillus cereus growth. Determination of amylase activity, depend on assay enzyme extract against standard curve of maltose.
Oseni OA, Ekperigin MM. Isolation and activity of alpha Amylase from selected bacteria strains in the Forest soil. Global J. Biosc. and Biotech. 2013;2:17-20.
Khedr MA, Emad A. Ewais, Khalil KMA. Improvement of thermophilic α-amylase productivity through UV mutagenesis and AmyE gene amplification and sequencing. Journal of Innovations in Pharmaceutical and Biological Sciences (JIPBS). 2017; 4(2):58-67.
Prasanth Manohar, Shanthini T, Kodiveri Muthukalianan Gothandam, Velu Rajesh Kannan, Nachimuthu Ramesh. Enhanced amylolytic activity of intracellulre α-amylase produced by bacillus tequilensis. J Microbiol Biotech Food Sci / Manohar et al. 2017;6(6):1314-1318.
Nisha Kumari, Veena Jain, Kamla Malik, Sushil. Production and optimization of amylase from Bacillus cereus using submerged fermentation. Int. J. Curr. Microbiol. App. Sci. 2017;6(6):263-271.
Fahad A. Al-Dhabaan. Morphological, biochemical and molecular identification of petroleum hydrocarbons biodegradation bacteria isolated from oil polluted soil in Dhahran, Saud Arabia. Saudi Journal of Biological Sciences. 2019;26:1247-1252.
Collee JG, Duguid JP, Fraser AG, Marmion BP. Practical medical microbiology. Edinburgh London Melbourne and New York. 1989;2.
Bharathiraja B, Jayakumar M, Nithyananthi MJT, Vinosh Muthukumar P, Saravanaraj A, Senthilkumar K. Production and kinetics of amylase from starch using mutant strain of Bacillus Sp MTCC. Journal of Chemical and Pharmaceutical Sciences JCPS. 1434; 9(1).
Sekar Sudharhsan, Sivaprakasam Senthilkumar, Karunasena Ranjith. Physical and nutritional factors affecting the production of amylase from species of bacillus isolated from spoiled food waste. African Journal of Biotechnology. 2007; 6(4):430-435.
Padhiar AR, Kommu S. Isolation, characterization and optimization of bacteria producing amylase. Int. J. Adv. Res. Biol. Sci. 2016;3(7):1-7.
Mushtaq Q, Irfan M, Tabssum F, Iqbal Qazi J. Potato peels: A potential food waste for amylase production. J Food Process Eng. 2017;40:e12512. Available:https://doi.org/ 10.1111/jfpe.12512
Tole SB, Fawade MM, Patil SM. Amylase activity of a starch degrading bacteria isolated from soil around tulja-bhavani temple of Tuljapur. IOSR Journal of Pharmacy and Biological Sciences. 2016; 11:2278-3008.
Rajani Chowdary A, Palkar Omkar Prakash, Walawalkar Ankita Kishor. Optimization of amylase production from Bacillus cereus using solid state fermentation. Journal of Biotechnology Research. 2018;4(8):58-65.
Agger T, Spohr AB, Nielsen J. α- Amylase production in high cell density submerged cultivation of Aspergillus oryzae and A. nidulans. Applied Microbiology and Biotechnology. 2001; 55(1):81-84.
Nisha Kumari, Veena Jain, Kamla Malik, Sushil. Production and optimization of amylase from bacillus cereus using submerged fermentation. Int. J. Curr. Microbiol. App. Sci. 2017;6(6):263-271.
Sivakumar T, Shankar T, Vijayabaskar P, Muthukumar J, Nagendrakannan E. Amylase production using Bacillus cereus isolated from a vermicompost site. International Journal of Microbiological Research. 2012;3(2):117-123.
Samrot A, Vijay A. Α-amylase activity of wild and mutant strains of Bacillus Sp. The Internet Journal of Microbiology. 2008; 6(2).