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Aim: To determine the anti-inflammatory activity of zinc oxide nanoparticles prepared using Punica granatum and Elettaria cardamomum.
Background: Over the counter and prescription non-steroidal anti-inflammatory agents like Aspirin, diclofenac, and ibuprofen are the most commonly used drugs for inflammation and its associated conditions. Unfortunately, these drugs are associated with many adverse side effects. Hence search for a more potent and safe alternative has been on the rise. In the current study zinc oxide nanoparticles known for its good anti-inflammatory activity has been prepared using a green method mediated by extract from Punica granatum and Elettaria cardamomum to reduce the side effects.
Materials and Methods: Collection and preparation of Punica granatum and Elettaria cardamomum extract, synthesis of zinc oxide nanoparticles, collection of nanoparticles using centrifugation, characterization of the nanoparticles, anti-inflammatory activity of ZnO nanoparticle using ultraviolet-visible spectroscopy, and inhibition of albumin denaturation assay were performed.
Results: Punica granatum and Elettaria cardamomum mediated zinc oxide nanoparticles showed good anti-inflammatory activity which was comparable to the control Diclofenac.
Conclusion: The data from the study has shown that Punica granatum and Elettaria cardamomum mediated zinc oxide nanoparticles have shown potent anti-inflammatory property and further research can be undertaken in order to subject it for various medicinal purposes.
Chen L, Deng H, Cui H, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2018;9:7204–7218.
Vane JR, Botting RM. Anti-inflammatory drugs and their mechanism of action. Inflammation Research. 1998;47:78–87.
Santhoshkumar J, Agarwal H, Menon S, et al. A biological synthesis of copper nanoparticles and its potential applications. Green Synthesis, Characterization and Applications of Nanoparticles. 2019;199–221.
Sirelkhatim A, Mahmud S, Seeni A, et al. Review on zinc oxide nanoparticles: Antibacterial activity and toxicity mechanism. Nano-Micro Letters. 2015;7: 219–242.
Nagajyothi PC, Cha SJ, Yang IJ, et al. Antioxidant and anti-inflammatory activities of zinc oxide nanoparticles synthesized using Polygala tenuifolia root extract. Journal of Photochemistry and Photobiology B: Biology. 2015;146:10–17.
Rajeshkumar S, Naik P. Synthesis and biomedical applications of cerium oxide nanoparticles- A review. Biotechnology Reports. 2018;17:1–5.
Kumar KHS, Dhananjaya N, Yadav LSR. E. tirucalli plant latex mediated green combustion synthesis of ZnO nanoparticles: Structure, photoluminescence and photo-catalytic activities; 2018. DOI: 10.1016/j.jsamd.2018.07.005
Rajeshkumar S, Bharath LV. Mechanism of plant-mediated synthesis of silver nanoparticles – A review on biomolecules involved, characterisation and antibacterial activity. Chemico-Biological Interactions 2017; 273: 219–227.
Menon S, Ks SD, R S, et al. Selenium nanoparticles: A potent chemotherapeutic agent and an elucidation of its mechanism. Colloids Surf B Biointerfaces. 2018;170: 280–292.
Agarwal H, Venkat Kumar S, Rajeshkumar S. A review on green synthesis of zinc oxide nanoparticles – An eco-friendly approach. Resource-Efficient Technologies. 2017;3:406–413.
Rajeshkumar S, Lakshmi T, Naik P. Chapter 18 - Recent advances and biomedical applications of zinc oxide nanoparticles. In: Shukla AK, Iravani S (eds) Green Synthesis, Characterization and Applications of Nanoparticles. Elsevier. 2019;445–457.
Abitha T, Santhanam A. Correlation between bizygomatic and maxillary central incisor width for gender identification. Brazilian Dental Science. 2019;22:458–466.
Alexander A, Ramani P, Sherlin H, et al. Quantitative analysis of copper levels in areca nut plantation area – A role in increasing prevalence of oral submucous fibrosis: An In vitro study. Indian Journal of Dental Research. 2019;30:261.
Jayaraj G, Herald J. Sherlin, Ramani P, et al. Malignant glomus tumour of the head and neck–A review. Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology. 2019;31:228–230.
Krishnan RP, Ramani P, Sherlin HJ, et al. Surgical specimen handover from operation theater to laboratory: A survey. Ann Maxillofac Surg. 2018;8:234–238.
Sisira Padavala GS. Molar incisor hypomineralization and its prevalence. Contemp Clin Dent. 2018;9:246.
Sridharan G, Ramani P, Patankar S, et al. Evaluation of salivary metabolomics in oral leukoplakia and oral squamous cell carcinoma. J Oral Pathol Med. 2019;48: 299–306.
Sujatha G, Muruganandan J, Vishnu Priya V, et al. Knowledge and attitude among senior dental students on forensic dentistry: A survey. World Journal of Dentistry. 2018;9:187–191.
Sujatha G, Muruganandhan J, Vishnu Priya V, et al. Determination of reliability and practicality of saliva as a genetic source in forensic investigation by analyzing DNA yield and success rates: A systematic review. Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology. 2019; 31:218–227.
Lin CK, Kazmierczak BI. Inflammation: A double-edged sword in the response to Pseudomonas aeruginosa infection. Journal of Innate Immunity. 2017;9:250–261.
Agarwal H, Shanmugam V. A review on anti-inflammatory activity of green synthesized zinc oxide nanoparticle: Mechanism-based approach. Bioorganic Chemistry. 2020;94:103423.
Patra JK, Das G, Fraceto LF, et al. Nano based drug delivery systems: Recent developments and future prospects. J Nanobiotechnology. 2018;16:1–33.
Auld DS. Zinc Coordination sphere in biochemical zinc sites. Biometals. 2001;14. DOI: 10.1023/a:1012976615056
Kim MH. Biological effects of zinc oxide nanoparticles on inflammation. Tang [Humanitas Medicine] 2016;6:23.1–23. 6.
Das RK, Pachapur VL, Lonappan L, et al. Biological synthesis of metallic nanoparticles: Plants, animals and microbial aspects. Nanotechnology for Environmental Engineering. 2017;2. DOI: 10.1007/s41204-017-0029-4
MS, Srinisha M, Rajeshkumar S, et al. Amla fruit mediated synthesis of zinc oxide nanoparticles and its antifungal activity. International Journal of Research in Pharmaceutical Sciences. 2019;10:2826–2829.
Maedeh Sadat Mohseni, Mohammad A. Khalilzadeh, Moein Mohseni et al. Green synthesis of Ag nanoparticles from pomegranate seeds extract and synthesis of Ag-Starch nanocomposite and characterization of mechanical properties of the films. Biocatalysis and Agricultural Biotechnology. 2020;25.
Sukri SNAM, Siti Nur Amalina, Shameli K, et al. Cytotoxicity and antibacterial activities of plant- mediated synthesized zinc oxide (ZnO) nanoparticles using Punica granatum (pomegranate) fruit peels extract. Journal of Molecular Structure. 2019; 1189:57–65.
Colombo E, Sangiovanni E, Dell’Agli M. A review on the anti-inflammatory activity of pomegranate in the gastrointestinal tract. Evidence-Based Complementary and Alternative Medicine. 2013;1–11.
Souissi M, Azelmat J, Chaieb K, et al. Antibacterial and anti-inflammatory activities of cardamom (Elettaria cardamomum) extracts: Potential therapeutic benefits for periodontal infections. Anaerobe. 2020;61:102089.