EFFICIENCY OF SYMBIOTIC NITROGEN FIXATION OF SOY NODULE BACTERIA AFTER PRESERVATION
ROXILA JURAEVA *
Institute of Microbiology, Tashkent, Uzbekistan
JAVLON TASHPULATOV
Institute of Microbiology, Tashkent, Uzbekistan
ABDUVALI IMINOV
Research Institute of Breeding, Seed Production and Agricultural Technology of Cotton Cultivation, Uzbekistan
XOLMUROD BOZOROV
Research Institute of Breeding, Seed Production and Agricultural Technology of Cotton Cultivation, Uzbekistan
LYUDMILA ZAYNITDINOVA
Institute of Microbiology, Tashkent, Uzbekistan
SVETLANA KUKANOVA
Institute of Microbiology, Tashkent, Uzbekistan
*Author to whom correspondence should be addressed.
Abstract
Viability of cells of nodule bacteria Bradyrhizobium japonicum after long term preservation in freeze-dried state and under the layer of mineral oil was studied. It was established that both tested methods of preservation cause no great difference in morphological and cultural features and result in preservation of stability by cells. Field trials revealed that inoculation of seeds by nodule bacteria on standard NPK background greatly enhanced growth, development and formation of nodules, and yield of soy (Orzu variety). The action of initial dose of nitrogen (30, 60, 90) on activity of the nitrogen fixation process and nitrogen nutrition of soy was studied.
Keywords: Nodule bacteria, soy, seeds, inoculation, mineral fertilizers, symbiosis.