ANTI BACTERIAL ACTIVITY OF Cordia dichotoma G. FORST. BARK HEXANE EXTRACT AND IDENTIFICATION OF ITS CONSTITUENTS BY GC-MS ANALYSIS
GONE SWAPNA
Department of Chemistry, University College of Sciences, Acharya Nagarjuna University, Nagarjunanagar-522510, Guntur Dt., Andhra Pradesh, India
KAKI SHIVA SHANKER
Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India
THIRUPATHI AZMEERA
Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India
G. USHA RANI
Department of Chemistry, University College of Sciences, Acharya Nagarjuna University, Nagarjunanagar-522510, Guntur Dt., Andhra Pradesh, India
BHATTIPROLU KESAVA RAO *
Department of Chemistry, University College of Sciences, Acharya Nagarjuna University, Nagarjunanagar-522510, Guntur Dt., Andhra Pradesh, India
*Author to whom correspondence should be addressed.
Abstract
Aim: Cordia dichotoma G. Forst belongs to the family Boraginaceae is known for its biological activities. The plant stem bark powder has been used to treat ulcers. The stem bark has anti-microbial and anti-implantation activities. The present study aims to analyse the phytoconstituents of stem bark hexane extract by GC-MS analysis, and to investigate its antibacterial activity.
Methods: The powdered stem bark was extracted with cold and hot hexane solvent and then the solvent was distilled to check its constituents by GCMS. The crude hexane was further purified by saponification, Column chromatography, and preparative TLC. The Fractions obtained from the above-mentioned methods were analyzed by GCMS. The hexane extract was further evaluated for antibacterial activity using two bacterial pathogens by the disc diffusion method.
Results: From GCMS analysis, eleven components were identified from crude hexane extract, eight components from Fraction 16c, and three components from Fraction 16d based on peak area percentage, retention time, and structure.
Keywords: Phyto sterols and column chromatography, preparative TLC, anti-bacterial activity.