ISOLATION OF ACTINOMYCETES FROM RHIZOSPHERE SOIL: A COMPLETE APPROACH
CHANDRASEN CHANDWAD *
Department of Microbiology, Mrs. Kesharbai Sonajirao Kshirsagar Alias Kaku Arts, Science and Commerce College, Beed, Pin 431122, Maharashtra, India
SHIVABAI CHANDWAD
Department of Microbiology, Mrs. Kesharbai Sonajirao Kshirsagar Alias Kaku Arts, Science and Commerce College, Beed, Pin 431122, Maharashtra, India
SUDHAKAR GUTTE
Department of Microbiology, Mrs. Kesharbai Sonajirao Kshirsagar Alias Kaku Arts, Science and Commerce College, Beed, Pin 431122, Maharashtra, India
*Author to whom correspondence should be addressed.
Abstract
Objectives: The current study focuses on the isolation of actinomycetes from various rhizosphere soil samples by using different pretreatments and selective medium.
Methods: A actinomycetes were isolated from rhizosphere soil samples of different plants by soil pretreatment, physical and chemical treatment and grown with and without an antibiotic medium.
Results: A total 69 of actinomycetes strains were isolated from rhizosphere soil samples by using physical and chemical pretreatment, including dry heat, centrifugation, phenol treatment, enrichment in a nutrient medium, calcium carbonate treatment, and selective nutrient medium with and without antibiotics. Physical treatment and chemical concentration effect the isolation of actinomycetes. It is found that dry heat at 40°C an effective pretreatment, centrifugation eliminates the unwanted soil debris, phenol and calcium carbonate are more effective chemical treatments, antibiotics medium is useful for isolation of actinomycetes by preventing bacterial and fungal growth. It is found that actinomycetes are rich in rhizosphere soil and have the ability of produce plant growth hormones, bio-nutrients, bioactive compounds that are useful to promote soil fertility and plant growth.
Keywords: Actinomycetes, antibiotics, calcium carbonate, rhizosphere, bioactive compounds, dry heat.