COMPARATIVE ANALYSIS OF CITRIC ACID PRODUCTION BY Aspergillus niger USING DIFFERENT MEDIA
AMOL BAPURAO KHANDAGALE *
Department of Biotechnology, Dayanand Science College, (DSCL) Latur, SRTM University, Nanded, Maharashtra, India
SHARAD CHANDRAKANT GANGAVANE *
Department of Biotechnology, Dayanand Science College, (DSCL) Latur, SRTM University, Nanded, Maharashtra, India
GAURI YOGENDRA KULKARNI
Department of Biotechnology, Dayanand Science College, (DSCL) Latur, SRTM University, Nanded, Maharashtra, India
GAURI SUHAS MANDLE
Department of Biotechnology, Dayanand Science College, (DSCL) Latur, SRTM University, Nanded, Maharashtra, India
VIJAY JAGDISH UPADHYE
Department of Microbiology, Parul Institute of Applied Sciences (PIAS), Parul University, Vadodara, Gujarat, India
*Author to whom correspondence should be addressed.
Abstract
Aspergillus niger was used for the submerged production of citric acid to explore the high yield citric acid producers other than citrus fruits. Rice and potato extracts were taken as substrates for a comparative analysis of higher yield giving substrate. Varying concentrations of Sucrose, Glucose and Nitrogen supplements were used to trace the fluctuations in yield of citric acid production. The citric acid extracted from variable media was titrated to determine the concentration of citric acid produced. The comparative study was carried out to find out the optimum requirements for the higher yield of citric acid production. This study can give a better approach for the large-scale industrial production of citric acid as it is among the highly consumed organic acids.
Keywords: Citric acid, fermentation, submerge fermentation, Aspergillus niger, potato, rice.