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Multidrug-resistant pathogens have promoted research of nontraditional antibiotic agents. Several classes of nano-antibiotics have affirmed their effectiveness against various eukaryotic microorganisms including bacteria, viruses and fungi. The production of silver nanoparticles using Penicillium expansum was investigated. Ag-nanoparticles were characterized by UV–visible spectrum, energy dispersive X-ray, zeta sizer Nano S90 and transmission electron microscopy. Biosynthesize Ag+ nanoparticles were confirmed by 420 nm corresponding to a surface plasmon resonance. EDX has confirmed the presence of the silver element. TEM micrographs showed irregular, spherical, hexagonal and monodispersed nanoparticles of 9–18 nm. Bio-Ag nanoparticles showed a significant antibacterial effect on Methicillin-Resistant Staphylococcus aureus RCMB 010010. The results showed a cytotoxicity activity on the human breast cancer (MCF-7) cell line in a concentration-dependent manner. Thus, biosynthesized Ag-nanoparticles are eco-friendly antibacterial and antitumor compounds.
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