DEVELOPMENT OF AN EFFICIENT PROTOCOL FOR TISSUE CULTURE OF SUGARCANE

Main Article Content

SHUBHANGI SALOKHE

Abstract

Availability of disease-free and genetically homogeneous planting material is essential for achieving a high yield in sugarcane cultivation. Tissue culture is one of the best techniques to produce disease-free, true-to-type, and vigorous plants of sugarcane which can be used as seed cane. This study aimed to develop an efficient protocol for the production of tissue-cultured plantlets of sugarcane, to decide suitable potting mix for hardening of these plantlets in the greenhouse, and to develop agronomy for growing them in the field for a three-tier system of sugarcane seed production. During this study, explants (meristems) from sugarcane variety ‘Co 86032’ were used from sugarcane seed nursery. These meristems were placed on Moorashige and Skoog (MS) medium supplemented with different combinations of cytokines and auxin. We observed multiple shoots on MS medium supplemented with BAP 0.2 mg/l and Kinetin 0.1 mg/l. Best rooting was observed on MS media supplemented with IAA 1 mg/l and NAA 1 mg/l. Soil mixture [Soil + Vermiculite + Sand in 4:1:1 proportion (by volume)] + Vermicompost @ 25% by volume of medium + NPK is best suited for hardening tissue-cultured plants in the greenhouse. The most appropriate method for planting tissue-cultured plantlets in the field was recognized as 2 feet distance between 2 plants and 3 feet distance between 2 rows. Setts obtained from micro-propagated plants (M) gave the highest yield as compared to setts from conventional sugarcane cultivation. Setts obtained from tissue-cultured plants planted at 15 cm spacing, gave a higher yield than those of other spacing. The protocol developed in this study shows potential for the development of tissue-cultured sugarcane plantlets and this will help to supply healthy seed cane to the sugarcane farmers, which is the main prerequisite for improving the productivity of the sugarcane crop.

 

Keywords:
Biotechnology, healthy seed, meristem culture, yield

Article Details

How to Cite
SALOKHE, S. (2021). DEVELOPMENT OF AN EFFICIENT PROTOCOL FOR TISSUE CULTURE OF SUGARCANE. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(27-28), 9-21. Retrieved from https://ikprress.org/index.php/PCBMB/article/view/6177
Section
Original Research Article

References

Goyal MR. Sustainable micro irrigation: principles and practices, Apple Academic Press, Canada; 2015.

Venkatesh D, Venkateswarlu M. An overview of Indian sugar industry. BIMS International Journal of Social Science Research. 2017;2(1):11–16.

Solomon S, Yang-Rui L. The sugar industry of Asian Region, Sugar Tech. 2016;16(6):557–558.

Dabbert S, Lewandowski I, Weiss J, Pyka A. Knowledge-driven developments in the bioeconomy: Technological and economic perspectives. Springer International Publishing, Switzerland; 2017.

Chakravarthi M. Sugarcane biotechnology: Challenges and prospects. Springer, Brazil. 2017;2.

Ibrahim M, Aman J, Negi T. Evaluation of tissue culture raised sugarcane planting materials against their donor conventional seed sources as initial source of seed cane at Tendaho Sugar Development Project, North-Eastern Ethiopia. Journal of Horti-culture. 2016;3(1):1¬–5.

Rithesh S. Comparative economics of sugarcane processed for sugar jaggery, Thesis submitted to the University of Agricultural Sciences, Dharwad in partial fulfilment of the requirements for the Degree of Master of Science (Agriculture); 2013.

Ali A, Naz S, Siddiqui F, Iqbal J. An efficient protocol for large scale production of sugarcane through micropropagation. Pakistan Journal of Botany. 2008;40(1): 139–149.

Sharma R, Tamta S. A review on red rot: The “Cancer “of sugarcane. J. Plant Pathol Microbiol. 2015;S1(003):1–8.

Kumar J, Saxena SC. Seed health management for better productivity, Proceedings of the 20th Training, Centre for advanced training in plant pathology, ICAR, New Delhi; 2008.

Hamza TA, Alebjo AL. Sugarcane (Saccharum officinarum L) tissue culture in Ethiopia: Opportunities for Ethiopia’s sugar industries. International Journal of Scientific and Technology Research. 2017; 6(8):398–406.

Panattoni A, Luvisi A, Triolo E. Review: Elimination of virus in plant: Twenty years of progress, Spanish Journal of Agricultural Research. 2013;11(1):173–188.

Walkey DGA. Production of virus-free plants by tissue culture. In Ingram, D.A. & Helgeson, J.P. (Eds.), Tissue culture methods for plant pathologists. Blackwell Oxford. 1980;109–117.

Srinivasan TV, Jalaja NC. Utilization of tissue culture technique in sugarcane improvement. C. Meristem culture. Annual Report. Sugarcane Breeding Institute, Coimbatore. 1981;68.

Jalaja NC. Micropropagation of sugarcane varieties for quality seed production. Manual on Sugarcane Production Technology. Sugarcane Breeding Institute, Coimbatore. 2001;48-52.

Lal N, Singh NH. Rapid clonal multiplication of sugarcane through tissue culture. Plant Tissue Cult. 1994;4(1):1– 7.

Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473–479.

Schaller GE, Street IH, Kiber JJ. Cytokinin and the cell cycle. Current Opinion in Plant Biotechnology. 2014;21:7-15.

Shukla R, Khan AQ, Gang GK. In vitro clonal multiplication of sugarcane; Optimization of media and hardening of plants. Sugar Tech. 1994;11(1):89– 94.

Geeta S, Padmanabhan D. Effect of hormones on direct somatic embryogenesis in sugarcane. Sugar Tech. 2001;3(3):120–121.

Tefsa M, Fitwi M. In vitro plant regeneration of sugarcane (Saccharum spp.) variety inoculated under different levels of plant growth regulators. J Plant Biochem Physiol. 2018;6(4):1–4.

OO KT, Htwe MM, San NN. In vitro regeneration of sugarcane (Saccharum officinarum) varieties GUI 11 and PMA 96/48. Journal of Scientific and Innovative Research. 2018;7(1):7–11.

Sabaz AK, Rashid H, Fayyaz CM, Chaudhry Z, Afroz A. Rapid micro-propagation of three elite Sugarcane (Saccharum officinarum L.) varieties by shoot tip culture. AJB. 2008;7(13):2174–2180.

Raghu S, Jayram S, Prabhakaran P, Vekatesalu V. Influence of spacing on growth and yield of sugarcane raised through in vitro micropropagation, Sugar Tech. 2006;8(1):82–84.

Tolera B, Shimelis D. Evaluation of tissue culture raised crops of sugarcane as initial source of seed cane at Matahara sugar estate, Ethiopia. Journal of Applied Biotechnology & Bioengineering. 2017; 3(2):299–303.

Ibrahim M, Aman J, Negi T. Evaluation of tissue culture raised sugarcane planting materials against their donor conventional seed sources as initial source of seed cane at Tendaho Sugar Development Project, North-Eastern Ethiopia. Journal of Horticulture. 2016;3(1):1¬–5.

Bailey RA, Bechet GR. A comparison of seed cane derived from tissue culture with conventional seed cane. Proceedings of the South African Sugar Technologists' Association. 1989;125–129.

Shezi SN, Ramburan S. Agronomic comparisons of sugarcane varieties derived from tissue culture (Nova Cane®) and conventional propagation under rain fed conditions. Journal of Crop Improvement. 2018;32(5):705–706.