MOLECULAR VARIABILITY OF DIFFERENT ISOLATES OF Rhizoctonia solani KUHN USING URP-PCR
RAO YADUMAN
Department of Plant Pathology, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj-211007 (UP), India.
ABHILASHA A. LAL
Department of Plant Pathology, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj-211007 (UP), India.
SHIVAM SINGH *
Department of Plant Pathology, School of Agriculture, Lovely Professional University, Phagwara-144411, Punjab, India.
*Author to whom correspondence should be addressed.
Abstract
Sheath blight disease, caused by Rhizoctonia solani, is an important fungal disease of rice (Oryza sativa L.). Twenty four isolates of R. solani, collected from different locations of Prayagraj (U.P.), India, were genetically characterized with URPs using PCR. For polymorphic fingerprint patterns, all primers were found effective from DNA of R. solani. Out of all used URPs, one primer (13R) produced a uniform DNA band in all isolates of R. solani. Results of this study shows that URPs showed reproducible results to assay genetic variability and are very sensitive.
Keywords: Genetic variability, Oryza sativa, Rhizoctonia solani, URP-PCR