CHARACTERIZATION AND QUALITY IMPROVEMENT OF MNH992 AND VH289 BT COTTON SEED VARIETIES

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SHRINIVASULU KAMMA
KAVYASRI ARUMILLI
SHAIK MOHAMMAD ANJUM
P. V. HEMANTH SAI
MOHITH PARIMI
VENGILI LAXMISAI DIXITHA
B. VIGNESHWARA REDDY
SURYANARAYANA VEERAVILLI

Abstract

In this study, we used PCR and ELISA techniques to identify the specific Cry1Ac gene and proteins in MNH992 and VH289 transgenic cotton plants. MNH992 was extracted at a concentration of 21.9ug/g and VH289 at a concentration of 17.37ug/g from seed protein. PCR analysis confirmed that the gene specific CRY1Ac F/R primer produced amplicons with sizes of 550bp for the Bt gene, 600bp for the nptII gene, and 200bp for the 35s promoter gene. The ELISA method was used to validate the developed assay because it required less equipment and took less time.

Keywords:
PCR, ELISA, MNH992, VH289, Cry1Ac, Cotton

Article Details

How to Cite
KAMMA, S., ARUMILLI, K., ANJUM, S. M., SAI, P. V. H., PARIMI, M., DIXITHA, V. L., REDDY, B. V., & VEERAVILLI, S. (2021). CHARACTERIZATION AND QUALITY IMPROVEMENT OF MNH992 AND VH289 BT COTTON SEED VARIETIES. PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 22(71-72), 195-200. Retrieved from https://ikprress.org/index.php/PCBMB/article/view/7266
Section
Original Research Article

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