Molecular Variability in Phaeoisariopsis personata Causing Late Tikka Leaf Spot of Ground Nut in Cuddalore District of Tamil Nadu
S. Sundaramoorthy *
Department of Plant Pathology, Faculty of Agriculture, Annamalai University, Chidambaram- 608 002, Tamil Nadu, India.
J. Johnny Subakar Ivin
Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Chidambaram- 608 002, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
An experiment was carried out at Department of plant pathology, Annamalai University, Tamil Nadu, India. To test the five isolates of Phaeoisariopsis personata from different regions of groundnut Vridhachalam-2 (VRI-2) variety that showed typical symptoms of late tikka leaf spot disease from Cuddalore district viz., Shivapuri (LT1), Srimushnam (LT2), Chaithiram (LT3), Parangipettai (LT4), and Kammapuram (LT5) were collected. Random Amplified Polymorphic DNA (RAPD) analysis was used to detect the variations among the isolates of P. personata. Pair wise genetic similarities between the different isolates of P. personata were estimated by DICE similarity coefficient. The Dendrogram results revealed that these five isolates showed 66 per cent to 80 per cent similarity coefficient. There is no congruence between the RAPD pattern and the virulence pattern of test isolates. The clustering in the RAPD dendrogram was not associated with the virulence.
Keywords: Groundnut, random amplified polymorphic DNA, Phaeoisariopsis personata, leaf spot