Molecular Characterization of Ralstonia mannitolilytica Isolated from the Gut of Brahmina coriacea Grubs Using 16S Ribosomal RNA Gene Sequence Analysis
Suresh Kumar Mahala *
Department of Entomology, Maharana Pratap University of Agriculture & Technology, Udaipur, Rajasthan (313001), India.
RS Chandel
Department of Entomology, College of Agriculture, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh (176062), India.
Abhishek Walia
Department of Microbiology, College of Basic Sciences, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh (176062), India.
Shweta Sagar
Division of Microbiology, Indian Agricultural Research Institute, New Delhi-110012, India.
Karthik R
Department of Entomology, College of Agriculture, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh (176062), India.
*Author to whom correspondence should be addressed.
Abstract
Ralstonia mannitolilytica is an emerging aerobic gram-negative bacterium causing infection among immune compromised patients. It has been isolated from the soil, water, human body and plant surfaces but this is the first report of Ralstonia mannitolilytica isolated from the grubs of Brahmina coriacea in the world. The gut microbiota was isolated from different ecotypes of B. coriacea, collected from various parts of Himachal Pradesh, India. Ralstonia mannitolilytica strain GMG5 was found from the grubs of Seobagh population. Ralstonia mannitolilytica was able to degrade cellulose in the Carboxy Methyl Cellulose (CMC) media, where the cellulolytic index was recorded to be 0.33. The Cellulolytic bacterial isolates were identified using morphological, biochemical and 16S ribosomal RNA gene sequence analysis.
Keywords: Brahmina coriacea, Ralstonia mannitolilytica, gut microbiota, cellulolytic index, 16S ribosomal RNA