PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Silver nanoparticles are playing potential role in various fields such as medical, environmental, food, cosmetics, consumer goods, electronics, textiles etc. Due to which their production is getting hype for their multiple usage. Silver nanoparticles (AgNPs) traditionally can be synthesized by chemical and physical methods. But in past few decades, biological methods are utilized for the green synthesis of AgNPs. This research article shows the green synthesis of AgNPs which have been synthesized from leafs & flowers extract of Rosa indica & Catharanthus roseus. It has been reported, Rosa indica &Catharanthus roseus have possible therapeutic uses in many severe & common diseases such as cancer, diabetes etc. As both plant species have antibacterial, antifungal, antineoplastic & anticancer properties. In this process the leaf extract and flower extract of Catharanthus roseus and Rosa indica has been used as reducing agent, which was mixed with silver nitrate (AgNO₃). Further, the characterization of synthesized AgNPs was done with the help of UV-Vis spectroscopy & Transmission Electron Microscopy (TEM). Based on these results the absorption spectra of Catharanthus roseus and Rosa indica were both compared to detect which had the most absorption peak. TEM analysis revealed NPs were spherical, big, segregated and of two sizes 19.2 nm and 15.2 nm and shows that the dried flower extract had potential to form huge, spherical and uncluttered nanoparticles better than that from leaf extract of the plant.