In silico STUDY OF OSMOTIC STRESS-RESPONSIVE PROTEINS IN CANOLA (Brassica napus L. cv. Sarigol)
REZA SHOKRI-GHARELO *
Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz 51666-16471, Iran.
ALI BANDEHAGH
Department of Plant Breeding and Biotechnology, University of Tabriz, Tabriz 51666-16471, Iran.
BIZHAN MAHMOUDI
Department of Animal science, Ramin Agriculture and Natural Resources University, Ahvaz, Iran.
*Author to whom correspondence should be addressed.
Abstract
The complexity of plant responses to abiotic stresses make difficult to manage and target special genes/proteins to be used in improving crop performances. Therefore, understanding and insight into molecular mechanisms recruited by plants under stressful conditions is essence. In this aim, Sarigol, a salt-sensitive cultivar of canola, on the basis of its differentially expressed proteins was studied in silico. The results indicated that the majority of induced proteins had molecular function of catalytic activity. Proteins with the function of the response to stress were underrepresented by Sarigol, whereas proteins involved in the cellular and metabolic process were overrepresented. Phylogenetic analysis divided the osmotic stress induced proteins into 4 groups and prediction of protein-protein interaction network illustrated two sets of interacted proteins, while most of proteins did not show any interactions. The results suggested that in the molecular level, Sarigol is unable to implement appropriate actions whose are observed in tolerant plants.
Keywords: Biological process, Cys/Met metabolism, abiotic stress, PPI network