FLOW CYTOMETRIC ESTIMATION OF LACTOTRANSFERRIN CONTENT IN BOVINE MILK SAMPLES
AMIT GUPTA *
Department of Immunology and Virology, Vidya Pratishthan’s School of Biotechnology (VSBT) Vidyanagari, Baramati, District Pune, India.
SUSHAMA R. CHAPHALKAR
Department of Immunology and Virology, Vidya Pratishthan’s School of Biotechnology (VSBT) Vidyanagari, Baramati, District Pune, India.
*Author to whom correspondence should be addressed.
Abstract
The aim of our present study was determine the lactotransferrin (also called as lactoferrin) content from whey protein in bovine milk samples using flow cytometry. For these studies, whey protein (200 µl) containing cells were stained with lactoferrin FITC (Fluorescein isothiocyanate) and evaluated the percentage of lactoferrin content in bovine milk samples and also observed the forward scatter (shape and size) and side scatter (granularity of the cell) using flow cytometer. The results showed that whey protein containing cells from bovine milk samples showed variation in the lactoferrin content (22.39 to 36.56%) where as lactoferrin capsule used as standard bind with FITC for these studies showed maximum amount of lactoferrin content (39 to 41.89%) as compared to whey protein containing cells of bovine milk samples which was evidenced through increased in the level of forward scatter but decreased in the level of side scatter. Overall, the results showed that whey protein of bovine milk samples expressed lactoferrin content which is determined through flow cytometric analysis.
Keywords: Whey protein, lactoferrin, flow cytometer, forward scatter, side scatter