ANTIMITOTIC AND ANTIPROLIFERATIVE ACTIVITY OF STEM BARK OF Oroxylum indicum
VANSHIKA SHRIVASTAVA
School of Studies in Microbiology, Jiwaji University, Gwalior – 474 011, Madhya Pradesh, India.
RICHA SIJORIA
National Research Institute for Ayurveda-Siddha Human Resource Development, Aamkho, Gwalior – 474 009, Madhya Pradesh, India.
YADU NANDAN DEY
National Research Institute for Ayurveda-Siddha Human Resource Development, Aamkho, Gwalior – 474 009, Madhya Pradesh, India.
NARENDRA KUMAR PANDEY
National Research Institute for Ayurveda-Siddha Human Resource Development, Aamkho, Gwalior – 474 009, Madhya Pradesh, India.
ANKUSH JADHAV
National Research Institute for Ayurveda-Siddha Human Resource Development, Aamkho, Gwalior – 474 009, Madhya Pradesh, India.
MANISH WANJARI *
National Research Institute for Ayurveda-Siddha Human Resource Development, Aamkho, Gwalior – 474 009, Madhya Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Background: Oroxylum indicum (Family- Bignoniaceae), commonly known as Shyonak, is a plant of Ayurvedic system of medicine known to be used ethnomedicinally for treatment of cancer in North-East region of India. The present study was aimed to evaluate antimitotic and antiproliferative potential of its stem bark. Materials and Methods: The hydro-alcoholic (50%) extract of stem bark of Oroxylum indicum was prepared and used in present study. Antimitotic activity was assessed in Allium cepa assay while antiproliferative effect was studied in yeast proliferation model. Methotrexate was used as standard anticancer agent.
Results: In Allium assay, hydroalcoholic extract of Oroxylum indicum (4, 5 and 6 mg/ml) and methotrexate (0.02 mg/ml) showed significant concentration dependant inhibitory influence against the dividing cells of Allium roots and decreased root growth and mitotic index as compared to control. Antiproliferative studies showed that hydroalcoholic extract of Oroxylum indicum (4, 5 and 6 mg/ml) and methotrexate (50 and 100 µg/ml) exhibited interruption in cell proliferation of Saccharomyces cerevisiae as observed by marked reduction in number of dividing cells and inhibition of cell viability compared to control. The results indicated the cytotoxic potential of extract due to antimitotic and antiproliferative effects. The effect may be attributed to the presence of flavonoids, alkaloids and phenolic compounds like baicalein, oroxylum A, chrysin.
Conclusion: In conclusion, the hydroalcoholic extract of stem bark of Oroxylum indicum exhibited antimitotic and antiproliferative activity and indicates cytotoxic potential of the plant extract against abnormal cell growth as like in cancer. The study substantiates its ethnomedicinal use in the treatment of cancer.
Keywords: Oroxylum indicum, Allium cepa assay, yeast proliferation model, antimitotic, antiproliferative