OPTIMIZATION OF EXTRACTION OF DNA FROM Brassica oleracea var. botrytis (Cauliflower) USING RESPONSE SURFACE METHODOLOGY
DJABER TAZDAÏT *
Department of Biochemistry and Microbiology, Faculty of Biological and Agronomical Sciences, Mouloud Mammeri University of Tizi-Ouzou, P.O.Box 17, RP 15000 Hasnaoua, Tizi-Ouzou, Algeria
ZIAD CHANANE
Department of Biochemistry and Microbiology, Faculty of Biological and Agronomical Sciences, Mouloud Mammeri University of Tizi-Ouzou, P.O.Box 17, RP 15000 Hasnaoua, Tizi-Ouzou, Algeria
IDIR KERKACHE
Department of Biochemistry and Microbiology, Faculty of Biological and Agronomical Sciences, Mouloud Mammeri University of Tizi-Ouzou, P.O.Box 17, RP 15000 Hasnaoua, Tizi-Ouzou, Algeria
RYM SALAH-TAZDAÏT
Department of Biochemistry and Microbiology, Faculty of Biological and Agronomical Sciences, Mouloud Mammeri University of Tizi-Ouzou, P.O.Box 17, RP 15000 Hasnaoua, Tizi-Ouzou, Algeria
*Author to whom correspondence should be addressed.
Abstract
In this study, response surface methodology (RSM) was undertaken to optimize and model the DNA extraction from Brassica oleracea var. botrytis (Cauliflower). The design variables were the volume of the lysis buffer; the volume of chloroform and the ratio between the volume of NaCl / ethanol (v/v) and the volume of the aqueous phase recovered after purification while response variables were DNA concentration and purity.
The results of this study estimated the effects of the three parameters on the specified responses, as well as the regression coefficients of the developed linear mathematical models. On the other hand, the experimental values of responses agreed with those predicted by the two models employed. The optimal values of the three parameters studied were also determined.
Keywords: Cauliflower, DNA, extraction, modeling, optimization, response surface methodology