AMELIORATING ROLE OF HEMIN AND KETOCONAZOLE ON THE HIPPOCAMPUS DAMAGE IN THYROIDECTOMIZED RATS
FATEN ZAHREN MOHAMED
Biochemistry Units, Department of Chemistry, Faculty of Science, Zagazig University, Egypt.
EHAB TOUSSON *
Department of Zoology, Faculty of Science, Tanta University, Egypt.
MOHAMED A. MAHMOUD
Department of Chemistry, Faculty of Science, Port Said University, Egypt.
SAMR MW. GHONIM
Department of Chemistry, Faculty of Science, Port Said University, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Objective: The thyroid hormones are recognized as key metabolic hormones that play a critical role in brain development, mediate important effects within the CNS throughout life and regulate the metabolism and functions of various neurotransmitters. The present work aimed to declare the effect of low thyroid hormone status on acetyl cholinesterase, oxidative stress and total antioxidant parameters and the impact of these biomarkers on the hippocampus of thyroidectomized rats. Also, the present study aimed to elucidate the role of hemin and ketoconazole (KTZ) supplementation as a HO inducer and inhibitor respectively.
Methods: Seventy male Wistar rats were divided into the following groups: G1, Control group; G2, Thyroidectomized group; G3, Sham operated group; G4, Thyroidectomized & Hemin group; G5, Thyroidectomized & KTZ group; G6, Sham operated & Hemin group and G7, Sham operated & KTZ group.
Results: Hypothyroidism is documented by significantly decreased and increased serum T3& TSH levels respectively in thyroidectomized rats. The results obtained revealed that oxidative stress due to hypothyroidism go has a detriment effect on Hippocampus tissue by depending on the result of malondialdhyde (MDA) that is valid as marker of tissue damage and there was a negative significant correlation between MDA and serum ferric reducing antioxidant power (FRAP) as marker for total antioxidant capacity (TAC).
Conclusions: Our results revealed that, hemin has an ameroliating role in hippocampus of thyroidectomized rats by decreasing oxidative stress occurred as a result of hypothyroidism.
Keywords: Thyroidectomy, hippocampus, hemeoxygenase, hemin, ketoconazole, oxidative stress