Journal of Biochemistry International

The present study was carried out to determine fermentation characteristics, metabolizable energy (ME), digestible organic matter (DOM) and short chain fatty acids (SCFA) of raisin grapevine leaves (L), grapevine leaves treated with 0.5% urea (U) and grapevine leaves treated with microwave (M) using gas production technique. Two fistulated wether with average BW of 45±2 kg were used. The data was analyzed using a completely random design. The incubation times were 2, 4, 6, 8, 12, 16, 24, 36, 48 and 72 h. The gas production of soluble and insoluble fractions (a + b) were 289, 317.09 and 246.66 mLg^-1DM and the rate of gas production prices (c) were 0.0155, 0.0170 and 0.0272 (%/h) for treatments L, U and M, respectively. ME, DOM and SCFA of grapevine leaves were obtained 16.40 mjkg^-1DM, 178.43% and 1.91 mlmol; and of grapevine leaves treated with 0.5% urea achieved 16.99 mjkg^-1DM, 182.3% and 1.95 mlmol; and of grapevine leaves treated with microwave were calculated 18.35 mjkg^-1DM, 192.91% and 2.2 mlmol, respectively. These showed statistically significant differences (P<0.05). The grapevine leaves treated with microwave (148.58 mlLg^-1DM) due to the production of more gas than grapevine leaves treated with 0.5% urea and grapevine leaves (134.27 and 128.84 mlLgr^-1DM) resulted low ME in this treatments compared to grapevine leaves treated with microwave.