Journal of Biochemistry International

The study of the effects of the eye drops used in the two types of a sterile compounds (carboxy methyl cellulose sodium 0.5% (CMCS 0.5%) and sodium hyaluronate 0.2% (SH0.2%)) using electrochemical method by cyclic voltammetry in the natural media at pH=6.8 which is the same as tear film pH. This study aimed to evaluate the activity of both eye drops (carboxy methyl cellulose sodium 0.5% (CMCS0.5%) and sodium hyaluronate 0.2% (SH0.2%)) and redox effects on the eyes in tear film resembling media and blood medium.

The results were given for the CMCS0.5% in normal saline as an electrolyte in the cell of cyclic voltammetric technique, two cathodic current peaks of CMCS0.5% at 250 mV and -250 mV, While the result of voltammogram in distill water single reduction current peak of CMCS0.5% at -600 mV. But in blood medium the CMCS0.5% shows reduction current peak at -900 mV. The same antioxidants current peak appeared in KCl as an electrolyte at -100 mV, so the CMCS0.5% acts as antioxidant in the eye lubricant on the tear film in human eye and in different electrolytes.

The other compound of sterile eye drop is SH0.2% which was studied in normal saline that showed two oxidation current peaks at 250 mV and 500 mV and two reduction current peaks at 400 mV and -500 mV. While in the blood medium, it made one oxidation current peak at 1mV and other reduction current peak at -750 mV.

The both CMCS0.5% and SH0.2% eye drops can be used properly as a safe sterile eye drop regarding the chemical composition as they act as antioxidant agents in the neutral medium as in the tear media of human eyes.