STUDY OF THE ANTIOXIDANT ACTIVITY OF THE EXTRACTS OF THE ACHENES OF Sylibum marianum L.
FATIMA ZOHRA BESSAM *
Faculty of Natural and Life Sciences, Djillali Liabes University, Sidi Bel Abbes, 22000, Algeria.
ZOHEIR MEHDADI
Laboratory of Plant Biodiversity, Conservation and Valorization, Faculty of Natural and Life Sciences, University of Sidi Bel Abbes, Algeria.
GÉRARD LIZARD
Laboratory Bio-Peroxil (EA 7270): Biochemistry of the Peroxisome, Lipid Metabolism and Inflammation, Faculty of Sciences, University of Bourgogne, Gabriel, F-21000, Dijon, France.
RIAD DJELLOULI
Faculty of Natural and Life Sciences, Djillali Liabes University, Sidi Bel Abbes, 22000, Algeria.
*Author to whom correspondence should be addressed.
Abstract
The antioxidant activity of four extracts obtained from seeds of Sylibum marianum L. by trapping technique of free radical DPPH (2,2-diphényl1-1picrylhydrazyl) revealed a big antioxidant power, especially for the ethyl acetate extract with inhibitory concentration (IC50) of 0.20 mg / ml. The chloroform extracts and butanol possess a moderate antioxidant capacity (IC50 of 0.97 mg / ml and IC50 of 1.04 mg / ml). However the residual aqueous extract is the least active (IC50 of 5.64 mg / ml). The extract ethyl acetate presents an anti-radical efficiency of 5.00 very close to that of the ascorbic acid is 5.88. The thin layer chromatography confirmed the presence of flavonoids. The quantitative analysis shows that the ethyl acetate extract is the richest in flavonoids with grades of about 39.64 μg catechin equivalent per gram of dry matter.
Keywords: Sylibum marianum L, seeds, flavonoids, thin layer chromatography (TLC), antioxidant capacity