PHYTOCHEMICAL SCREENING AND TOTAL TANNIN CONTENTS OF EXTRACTS Sargassum polycystum FROM DUTUNGAN ISLAND
F. J. SAMI
Department of Chemistry, Hasanuddin University, Makassar, Indonesia. and Sekolah Tinggi Ilmu Farmasi, Makassar, Indonesia.
N. H. SOEKAMTO *
Department of Chemistry, Hasanuddin University, Makassar, Indonesia.
. FIRDAUS
Department of Chemistry, Hasanuddin University, Makassar, Indonesia.
J. LATIP
School of Chemical Sciences and Food Technology, The National University of Malaysia, Malaysia.
*Author to whom correspondence should be addressed.
Abstract
Quantitative profiling of total tannin contents of Sargassum polycystum from Dutungan island have been done. This research method uses folin ciocalteu reagent as measured with visible spectrophotometer. Extraction was carried out in stages using various solvent chloroform (non-polar), ethyl acetate (semi-polar) and methanol (polar). The results showed extract contained various kinds of secondary metabolites, the highest total tannin content was found in Sargassum polycystum extract of ethyl acetate solvent which was 6.678 mg ETA /g, followed by methanol which was 2.196 mg ETA/g, and the lowest total phenolic in chloroform extract was 1.5795 mg ETA /g. This total tannin can also be related to the antioxidant and other bioactivity.
Keywords: Total tannin, phytochemical screening, Sargassum polycystum, extracts, dutungan island.